Recombinant human TIGIT protein (Fc Chimera Active) (ab224646)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 92% SDS-PAGE
- Endotoxin level:
- Active: Yes
- Suitable for: SDS-PAGE, Functional Studies
Preparation and Storage
- DKFZp667A205
- ENSMUSG00000071552
- FLJ39873
Images
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SDS-PAGE - Recombinant human TIGIT protein (Fc Chimera Active) (ab224646)Human TIGIT, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
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Functional Studies - Recombinant human TIGIT protein (Fc Chimera Active) (ab224646)FACS assay shows that recombinant Human TIGIT Protein, Fc Tag can bind to 293T cell overexpressing human CD155. The concentration of TIGIT used is 0.1 µg/ml. -
Functional Studies - Recombinant human TIGIT protein (Fc Chimera Active) (ab224646)Immobilized Human CD155, mouse IgG2a Fc tag, low endotoxin at 5 µg/mL (100 µL/well) can bind Human TIGIT, Fc Tag with a linear range of 0.6-5 ng/mL. -
SDS-PAGE - Recombinant human TIGIT protein (Fc Chimera Active) (ab224646)ab224646 analyzed by SDS-PAGE under reducing conditions and stained overnight with Coomassie Blue.
The protein migrates as 40-55 kDa under reducing conditions due to glycosylation.
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Functional Studies - Recombinant human TIGIT protein (Fc Chimera Active) (ab224646)Immobilized ab224646 at 2 μg/ml (100 μl/well) can bind biotinylated human CD155, Fc Tag with a linear range of 5-78 ng/ml.
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Functional Studies - Recombinant human TIGIT protein (Fc Chimera Active) (ab224646)Immobilized ab224646 at 10 μg/ml (100 μl/well) can bind biotinylated human Nectin-2, Fc Tag with a linear range of 0.313-10 μg/ml.
