Recombinant human SMURF1 protein (ab95928)
Key features and details
- Expression system: Baculovirus
- Active: Yes
- Tags: DDDDK tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies
-
Product name
Recombinant human SMURF1 protein
See all SMURF1 proteins and peptides -
Biological activity
Specific Activity: 0.0055 pmol/min/µg Assay conditions: 50 µl reaction mix (4mM ATP, 5mM MgCl2, 1mM DTT, E1 (0.5 µg), E2 (2.8 µg), Biotin-Ubiquitin (0.5 µg) and SMURF1 (0-1 µg) in 50mM Tris pH 7.4) incubate for 30min at 37°C, then add to streptavidin coated plates and incubate for 30min at 37°C. Add antibody against SMURF1, incubate 1h. Finally, incubate 1h with the Europeum-labeled secondary antibody. Time resolved fluorescence measured at room temperature (Lambda exc=330 nm, Lambda em=620 nm). -
Purity
80 % .
Purity is >80% by SDS-PAGE. -
Expression system
Baculovirus -
Accession
-
Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
-
Species
Human -
Predicted molecular weight
70 kDa including tags -
Amino acids
150 to 757 -
Tags
DDDDK tag N-Terminus
-
Preparation and Storage
-
Stability and Storage
Shipped on Dry Ice. Store at -80°C.
pH: 8.00
Constituents: 0.0462% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.395% Tris HCl, 0.05% Tween, 20% Glycerol (glycerin, glycerine), 0.58% Sodium chloride
101 µg/ml FLAG peptideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
Images
-
Functional Studies - Recombinant human SMURF1 protein (ab95928)Image showing specific activity of ab95928.
-
SDS-PAGE - Recombinant human SMURF1 protein (ab95928)10% SDS-PAGE
Lane 1: ab95928 (3µg) Lane 2: Molecular weight markers
