Recombinant human PLGF protein (Active) (ab174025)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level:
- Active: Yes
- Tags: His tag C-Terminus
- Suitable for: SDS-PAGE, Functional Studies
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at 4°C prior to reconstitution. Store at -20°C or -80°C. Please see notes section.
pH: 7.4
Constituents: 0.6% Acetic acid, 10% Trehalose
pH 2-3This product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionReconstitute with 100mM acetic acid to a concentration of 200 µg/ml.
Images
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Functional Studies - Recombinant human PLGF protein (Active) (ab174025)
Immobilized ab174025 at 1 μg/mL (100 μL/well) can bind Biotinylated Human VEGF R1, His,Avitag (ab246160) with a linear range of 2-20 ng/mL
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Functional Studies - Recombinant human PLGF protein (Active) (ab174025)Immobilized Rhesus macaque VEGF R1, Mouse IgG2a Fc Tag, low endotoxin at 5 μg/mL (100 μL/well) can bind ab174025 with a linear range of 5-40 ng/mL
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SDS-PAGE - Recombinant human PLGF protein (Active) (ab174025)SDS-PAGE analysis of reduced ab174025 stained overnight with Coomassie Blue.
DTT-reduced Protein migrates as 28-33 kDa due to glycosylation.
