Antibodies, Proteins, Kits and Reagents for Life Science

Key features and details

Expression system: Escherichia coli
Purity: > 95% SDS-PAGE
Endotoxin level:
Suitable for: SDS-PAGE, HPLC

Product name

Recombinant Human PEA15 protein
See all PEA15 proteins and peptides
Purity

> 95 % SDS-PAGE.
ab172828 is greater than 95% pure, as determined by SEC-HPLC and reducing SDS-PAGE.
Endotoxin level
Expression system

Escherichia coli
Accession

Q15121
Protein length

Full length protein
Animal free

No
Nature

Recombinant
- Species
  
  Human
- Sequence
  
  GSHMAEYGTLLQDLTNNITLEDLEQLKSACKEDIPSEKSEEITTGSAWFS FLESHNKLDKDNLSYIEHIFEISRRPDLLTMVVDYRTRVLKISEEDELDT KLTRIPSAKKYKDIIRQPSEEEIIKLAPPPKKA
- Predicted molecular weight
  
  15 kDa
- Amino acids
  
  1 to 130

Our Abpromise guarantee covers the use of ab172828 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Applications

SDS-PAGE

HPLC
Form

Liquid
Concentration information loading...

Stability and Storage

Shipped on Dry Ice. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

pH: 7.40
Constituent: 100% PBS

Supplied as a 0.2 µM filtered solution

Alternative names
- 15 kDa phosphoprotein enriched in astrocytes
- Astrocytic phosphoprotein PEA 15
- Astrocytic phosphoprotein PEA-15
- Astrocytic phosphoprotein PEA15
- HMAT 1
- HMAT1
- Homolog of mouse MAT 1 oncogene
- Homolog of mouse MAT1 oncogene
- HUMMAT 1H
- HUMMAT1H
- MAT 1
- MAT 1H
- MAT1
- MAT1H
- PEA 15
- Pea15
- PEA15 protein
- PEA15_HUMAN
- PED
- Phosphoprotein enriched in astrocytes 15
- Phosphoprotein enriched in astrocytes 15kD
- Phosphoprotein enriched in diabetes
see all
Function

Blocks Ras-mediated inhibition of integrin activation and modulates the ERK MAP kinase cascade. Inhibits RPS6KA3 activities by retaining it in the cytoplasm (By similarity). Inhibits both TNFRSF6- and TNFRSF1A-mediated CASP8 activity and apoptosis. Regulates glucose transport by controlling both the content of SLC2A1 glucose transporters on the plasma membrane and the insulin-dependent trafficking of SLC2A4 from the cell interior to the surface.
Tissue specificity

Ubiquitously expressed. Most abundant in tissues such as heart, brain, muscle and adipose tissue which utilize glucose as an energy source. Lower expression in glucose-producing tissues. Higher levels of expression are found in tissues from individuals with type 2 diabetes than in controls.
Sequence similarities

Contains 1 DED (death effector) domain.
Post-translational
modifications

Phosphorylated by protein kinase C and calcium-calmodulin-dependent protein kinase. These phosphorylation events are modulated by neurotransmitters or hormones.
Cellular localization

Cytoplasm. Associated with microtubules.
Target information above from: UniProt accession Q15121 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols