Recombinant human PDE6C protein (Active) (ab271682)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: >= 5% SDS-PAGE
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
-
Product name
Recombinant human PDE6C protein (Active)
See all PDE6C proteins and peptides -
Biological activity
Specific Activity: ≥ 3,000 pmol/min/µg.Unit Definition: One unit is defined as the amount of enzyme that will convert 1 pmole of 3', 5'-cGMP to 5'-GMP per minute at 37°C.Assay Conditions: 10 mM Tris-HCl, pH 7.4, 10 mM MgCl2, 0.1 mg/ml BSA, 0.05% Tween-20, 200 µM cGMP, 2.5 kU 5'-nucleotidase, and serial dilutions of PDE6C at 37°C for 60 min. Quantified by 5'- nucleotidase cleaving the 5' -GMP product and releasing the phosphate group which is detected by Malachite Green Reagent. -
Purity
>= 5 % SDS-PAGE. -
Expression system
Baculovirus infected Sf9 cells -
Accession
-
Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
-
Species
Human -
Predicted molecular weight
125 kDa including tags -
Amino acids
1 to 855 -
Tags
GST tag N-Terminus -
Additional sequence information
Mature chain.
-
Preparation and Storage
- 5''-cyclic phosphodiesterase subunit alpha''
- cGMP phosphodiesterase 6C
- COD4
Images
-
Functional Studies - Recombinant human PDE6C protein (Active) (ab271682)
Specific activity of ab271682 was ≥ 3,000 pmol/min/µg.
-
SDS-PAGE - Recombinant human PDE6C protein (Active) (ab271682)SDS-PAGE analysis of 2 µg ab271682.