cAMP Assay Kit (Competitive ELISA, Fluorometric) (ab138880)
Key features and details
- Assay type: Competitive
- Detection method: Fluorescent
- Platform: Microplate reader
- Assay time: 3 hr
- Sample type: Cell culture media, Cell Lysate, Plasma, Tissue Extracts, Urine
- Sensitivity: 1 nM
Overview
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Product name
cAMP Assay Kit (Competitive ELISA, Fluorometric)
See all cAMP kits -
Detection method
Fluorescent -
Sample type
Urine, Plasma, Tissue Extracts, Cell Lysate, Cell culture media -
Assay type
Competitive -
Sensitivity
1 nM -
Assay time
3h 00m -
Product overview
cAMP Assay Kit ab138880 uses a sensitive fluorometric competitive ELISA method to quantify cAMP (cyclic AMP) levels in samples from mammals and other species.
The cAMP assay protocol uses a 96-well plate supplied coated with an anti-cAMP antibody.
HRP-labeled cAMP, and free cAMP within a sample, are added to each well of the plate. The cAMP assay is based on the competition between the HRP-labeled cAMP and the free cAMP for the fixed number of cAMP antibody binding sites on the plate.
The more free cAMP is present, the less HRP-labeled cAMP is bound. After a washing step, the amount of bound HRP-labeled cAMP is detected using a fluorometric HRP substrate. The level of signal is compared to a standard curve for free cAMP, which is run at the same time as the samples.
Unlike other cAMP assay protocols, this kit does not require an acetylation step to achieve the maximum sensitivity.
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Notes
This product was previously called cAMP Direct Immunoassay Detection Kit (Fluorometric).
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Platform
Microplate reader
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10 x 96 tests 10X Wash Solution 1 x 10ml 1 x 100ml AbRed Indicator 1 vial 1 vial Anti-cAMP-coated 96-well plate 1 unit 1 x 10 units Assay Buffer 1 x 20ml 1 x 100ml cAMP Standard 1 x 33µg 1 x 33µg Cell Lysis Buffer 1 x 10ml 1 x 100ml HRP-cAMP Conjugate 1 vial 1 vial Hydrogen Peroxide Solution 1 x 50µl 1 x 250µl Substrate Buffer 1 x 10ml 1 x 100ml -
Research areas
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Relevance
Adenosine 3’,5’-cyclic monophosphate (cyclic AMP, cAMP) is an important “second messenger” involved in many physiological processes. It plays a key role as an intracellular second messenger for transduction events that follow a number of extracellular signals. -
Alternative names
- Adenosine 3' 5' cyclic monophosphate
- Adenosine 3’ 5’ cyclic monophosphate
- Cyclic adenosine monophosphate
- Cyclic AMP
Images
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Sample Standard Curve for cAMPcAMP dose response was measured with ab138880 in a solid black 96-well plate with a fluorescence microplate reader. The kit can detect as low as 1 nM cAMP in a 100 µl reaction volume.
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Immunoassay - 138880 cAMP Direct Immunoassay Kit (Fluorometric)cAMP dose response in various amounts of HeLa cells after and without forskolin treatment (100 micromolar - ab120058)
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Immunoassay - cAMP Direct Immunoassay (fluorometric) (ab138880)cAMP dose response in various amounts of 3T3-L1 cells after and without forskolin treatment (100 micromolar - ab120058)
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Immunoassay - ab13880cAMP Direct Immunoassay Kit (fluorometric)cAMP concentration (nM) measured in various biological fluids following assay protocol.
