Anti-EPCR/CD201 antibody (ab56689)
Key features and details
- Mouse monoclonal to EPCR/CD201
- Suitable for: IHC-P, Flow Cyt, WB, ICC/IF
- Reacts with: Human, Recombinant fragment
- Isotype: IgG1
Overview
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Product name
Anti-EPCR/CD201 antibody
See all EPCR/CD201 primary antibodies -
Description
Mouse monoclonal to EPCR/CD201 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P HumanWB Recombinant fragment
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Immunogen
Recombinant full length protein, corresponding to amino acids 1-239 of Human EPCR/CD201
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General notes
This product was changed from ascites to tissue culture supernatant on 15 May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.4 -
Concentration information loading... -
Purity
Tissue culture supernatant -
Purification notes
Purified from TCS. -
Clonality
Monoclonal -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
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Flow Cytometry - Anti-EPCR/CD201 antibody (ab56689)
Overlay histogram showing A549 cells stained with ab56689 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab56689, 0.01μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
This image was generated using the ascites version of the product.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPCR/CD201 antibody (ab56689)EPCR/CD201 antibody (ab56689) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human malignant lymphoma, diffuse large B tissue.
This image was generated using the ascites version of the product.
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Western blot - Anti-EPCR/CD201 antibody (ab56689)Western blot against tagged recombinant protein immunogen using ab56689 EPCR/CD201 antibody at 1ug/ml. Predicted band size of immunogen is 52 kDa
This image was generated using the ascites version of the product.
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Immunocytochemistry/ Immunofluorescence - Anti-EPCR/CD201 antibody (ab56689)Immunofluorescence analysis of HeLa cells, staining EPCR/CD201 with ab56689 at 10 μ/ml.
This image was generated using the ascites version of the product.
