Recombinant human KDM4B / JMJD2B protein (ab198159)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: >= 68% SDS-PAGE
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies
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Product name
Recombinant human KDM4B / JMJD2B protein
See all KDM4B / JMJD2B proteins and peptides -
Biological activity
Specific Activity: ≥2.9 pmol/min/µg.
Assay conditions: 10 µl reaction mix containing assay buffer with 20 mM HEPES (pH 7.4), 50 mM NaCl, 1mM TCEP, 500 µM α-ketoglutarate, 25 µM iron, 2mM ascorbic acid, 0.01% Tween-20, 0.5 µM biotinylated peptide substrate, and JMJD2B (2 – 10ng) added to the wells. Add antibody against di-methylated K9 peptide. Incubate for 30 min, then Streptavidin-conjugated secondary antibody followed by Alpha Screening detection.
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Purity
>= 68 % SDS-PAGE. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
GSEDHGAQNPSCKIMTFRPTMEEFKDFNKYVAYIESQGAHRAGLAKIIPP KEWKPRQTYDDIDDVVIPAPIQQVVTGQSGLFTQYNIQKKAMTVGEYRRL ANSEKYCTPRHQDFDDLERKYWKNLTFVSPIYGADISGSLYDDDVAQWNI GSLRTILDMVERECGTIIEGVNTPYLYFGMWKTTFAWHTEDMDLYSINYL HFGEPKSWYAIPPEHGKRLERLAIGFFPGSSQGCDAFLRHKMTLISPIIL KKYGIPFSRITQEAGEFMITFPYGYHAGFNHGFNCAESTNFATLRWIDYG KVATQCTCRKDMVKISMDVFVRILQPERYELWKQGKDLTVLDHTRPTALT SPELSSWSASRASLKAKLLRRSHRKRSQPKKPKPEDPKFPGEGTAGAALL EEAGGSVKEEAGPEVDPEEEEEEPQPLPHGREAEGAEEDGRGKLRPTKAK SERKKKSFGLLPPQLPPPPAHFPSEEALWLPSPLEPPVLGPGPAAMEES -
Molecular weight information
83 kDa by SDS-PAGE -
Amino acids
2 to 500 -
Tags
GST tag N-Terminus
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Preparation and Storage
- JHDM3B
- JmjC domain-containing histone demethylation protein 3B
- JMJD2B
Images
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Functional Studies - Active human KDM4B / JMJD2B protein fragment (ab198159)
Specific activity of ab198159 was 0.32 pmole/min/µg.
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SDS-PAGE - Active human KDM4B / JMJD2B protein fragment (ab198159)SDS-PAGE analysis of ab198159.