Recombinant human IL-6 protein (Biotin) (ab208325)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level:
- Active: Yes
- Suitable for: Functional Studies, WB
Preparation and Storage
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Alternative names
- Interleukin BSF 2
- B cell differentiation factor
- B cell stimulatory factor 2
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Function
Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoeitic progenitor cells and cells of the CNS. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance. -
Involvement in disease
Genetic variations in IL6 are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ) [MIM:604302]. An inflammatory articular disorder with systemic-onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis.
Note=A IL6 promoter polymorphism is associated with a lifetime risk of development of Kaposi sarcoma in HIV-infected men. -
Sequence similarities
Belongs to the IL-6 superfamily. -
Post-translational
modificationsN- and O-glycosylated. -
Cellular localization
Secreted. - Information by UniProt
Images
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Functional Studies - Recombinant human IL-6 protein (Biotin) (ab208325)
Biotinylated Human IL-6, epitope tag free, primary amine labeling (ab208325) stimulates proliferation of TF-1 human erythroleukemic cell line. The EC50 for this effect is 0.2532-0.4489 ng/mL
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SDS-PAGE - Recombinant human IL-6 protein (Biotin) (ab208325)SDS-PAGE analysis of reduced ab208325 stained overnight with Coomassie Blue.
As a result of glycosylation, the reducing (R) protein migrates as 24-27 kDa in SDS-PAGE.
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ELISA - Recombinant human IL-6 protein (Biotin) (ab208325)Immobilized Human IL-6 R alpha, Fc Tag at 5 μg/mL (100 μL/well) can bind Biotinylated Human IL-6, Tag Free, ab208325 with a linear range of 1-20 ng/mL.
