Astana Biomed Group, an authorized Abcam distributor in Central Asia

Recombinant Human HUS1 protein (ab152054)

Key features and details

  • Expression system: Escherichia coli
  • Purity: > 95% SDS-PAGE
  • Endotoxin level:
  • Suitable for: SDS-PAGE

Description

  • Product name

    Recombinant Human HUS1 protein
    See all HUS1 proteins and peptides

  • Purity

    > 95 % SDS-PAGE.
    Purity is greater than 95% as determined by reducing SDS-PAGE. ab152054 is 0.2 µM filtered.

  • Endotoxin level

  • Expression system

    Escherichia coli

  • Accession

    O60921

  • Protein length

    Full length protein

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Sequence

      MKFRAKIVDGACLNHFTRISNMIAKLAKTCTLRISPDKLNFILCDKLANG GVSMWCELEQENFFNEFQMEGVSAENNEIYLELTSENLSRALKTAQNARA LKIKLTNKHFPCLTVSVELLSMSSSSRIVTHDIPIKVIPRKLWKDLQEPV VPDPDVSIYLPVLKTMKSVVEKMKNISNHLVIEANLDGELNLKIETELVC VTTHFKDLGNPPLASESTHEDRNVEHMAEVHIDIRKLLQFLAGQQVNPTK ALCNIVNNKMVHFDLLHEDVSLQYFIPALS

    • Predicted molecular weight

      32 kDa

    • Amino acids

      1 to 280

    • Specifications

    Our Abpromise guarantee covers the use of ab152054 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      SDS-PAGE

    • Form

      Liquid
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.

      pH: 8.00
      Constituents: 0.32% Tris HCl, 40% Glycerol, 0.58% Sodium chloride

    General Info

    • Alternative names

      • Checkpoint protein HUS1
      • hHUS1
      • Hus1
      • HUS1 (S. pombe) checkpoint homolog
      • HUS1 checkpoint homolog (S. pombe)
      • HUS1 Checkpoint Protein
      • HUS1 protein
      • HUS1+ - like protein
      • HUS1_HUMAN
      • Hydroxyurea-sensitive 1, S. pombe, homolog of
      see all

    • Function

      Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates.

    • Tissue specificity

      Ubiquitous.

    • Sequence similarities

      Belongs to the HUS1 family.

    • Cellular localization

      Nucleus. Cytoplasm. In discrete nuclear foci upon DNA damage. According to PubMed:14500360, localized also in the cytoplasm. DNA damage induces its nuclear translocation. Shuttles between the nucleus and the cytoplasm.
    • Information by UniProt

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab152054? Please let us know so that we can cite the reference in this datasheet.

ab152054 has not yet been referenced specifically in any publications.

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.

    pH: 8.00
    Constituents: 0.32% Tris HCl, 40% Glycerol, 0.58% Sodium chloride

Images

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