Recombinant human Flt3 / CD135 (mutated D835Y) protein (Active) (ab268580)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 70% SDS-PAGE
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
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Product name
Recombinant human Flt3 / CD135 (mutated D835Y) protein (Active)
See all Flt3 / CD135 proteins and peptides -
Biological activity
The specific activity of FLT3 (D835Y) was determined to be 46 nmol/min/mg in a kinase assay using abltide synthetic peptide substrate.
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Purity
> 70 % SDS-PAGE. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
RYESQLQMVQVTGSSDNEYFYVDFREYEYDLKWEFPRENLEFGKVLGSGA FGKVMNATAYGISKTGVSIQVAVKMLKEKADSSEREALMSELKMMTQLGS HENIVNLLGACTLSGPIYLIFEYCCYGDLLNYLRSKREKFHRTWTEIFKE HNFSFYPTFQSHPNSSMPGSREVQIHPDSDQISGLHGNSFHSEDEIEYEN QKRLEEEEDLNVLTFEDLLCFAYQVAKGMEFLEFKSCVHRDLAARNVLVT HGKVVKICDFGLARYIMSDSNYVVRGNARLPVKWMAPESLFEGIYTIKSD VWSYGILLWEIFSLGVNPYPGIPVDANFYKLIQNGFKMDQPFYATEEIYI IMQSCWAFDSRKRPSFPNLTSFLGCQLADAEEAMYQNVDGRVSECPHTYQ NRRPFSREMDLGLLSPQAQVEDS -
Actual molecular weight
73 kDa -
Amino acids
571 to 993 -
Modifications
mutated D835Y -
Tags
GST tag N-Terminus -
Additional sequence information
GenBank: NM_004119
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Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
pH: 7.50
Constituents: 0.79% Tris HCl, 0.87% Sodium chloride, 0.31% Glutathione, 0.003% EDTA, 0.004% DTT, 0.002% PMSF, 25% Glycerol (glycerin, glycerine)This product is an active protein and may elicit a biological response in vivo, handle with caution.
Images
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SDS-PAGE - Recombinant human Flt3 / CD135 (mutated D835Y) protein (Active) (ab268580)
SDS-PAGE analysis of ab268580.
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Functional Studies - Recombinant human Flt3 / CD135 (mutated D835Y) protein (Active) (ab268580)The specific activity of FLT3 (D835Y) was determined to be 46 nmol/min/mg in a kinase assay using abltide synthetic peptide substrate.
