Antibodies, Proteins, Kits and Reagents for Life Science

Anti-Bad (phospho S112) antibody [BADS112-B9] (ab278558)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [BADS112-B9] to Bad (phospho S112)
Suitable for: Flow Cyt
Reacts with: Mouse, Rat, Human

Product name

Anti-Bad (phospho S112) antibody [BADS112-B9]
See all Bad primary antibodies
Description

Rabbit monoclonal [BADS112-B9] to Bad (phospho S112)
Host species

Rabbit
Tested Applications & Species

Application Species
Flow Cyt
Human

See all applications and species data
Immunogen

Synthetic peptide within Human Bad (phospho S112). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q92934

Run BLAST with Run BLAST with
Positive control
- Flow cyt: U937 cells (treated with Calyculin A); C6 cells (treated with pervanadate); NIH/3T3 cells
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.

Application	Species
Flow Cyt	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.02% Sodium azide
Constituents: 49.88% PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA
Concentration information loading...
Purity

Protein A/G purified
Clonality

Monoclonal
Clone number

BADS112-B9
Isotype

IgG
Light chain type

lambda
Research areas

Flow Cytometry - Anti-Bad (phospho S112) antibody [BADS112-B9] (ab278558)

ab278558 recognizes basal phosphorylation levels in mouse cells. Flow cytometric analysis of NIH3T3 cells secondary antibody only (blue) or 0.1 µg/mL of isotype control (orange) or of ab278558 (green).
Flow Cytometry - Anti-Bad (phospho S112) antibody [BADS112-B9] (ab278558)

Peptide blocking flow cytometric analysis of U937 cells secondary antibody only negative control (blue) or Calyculin A-treated (green) or CalA and blocked with phospho peptide (gold) or CalA and blocked with non-phospho peptide (purple) using ab278558 at 0.1 µg/mL.
Flow Cytometry - Anti-Bad (phospho S112) antibody [BADS112-B9] (ab278558)

Flow cytometric analysis of U937 cells secondary antibody only negative control (blue) or untreated (red) or treated with Calyculin A (green) using ab278558 at 0.01µg/mL.
Flow Cytometry - Anti-Bad (phospho S112) antibody [BADS112-B9] (ab278558)

Flow cytometric analysis of C6 cells secondary antibody only negative control (blue), or treated with imatinib (red) or with pervanadate (green) using ab278558 at 0.08µg/mL.
Anti-Bad (phospho S112) antibody [BADS112-B9] (ab278558)

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