Anti-Bad (phospho S112) antibody [BADS112-B9] (ab278558)
![Anti-Bad (phospho S112) antibody [BADS112-B9] (ab278558)](https://www.abcam.com/ps/products/278/ab278558/Images/ab278558-4-bads112b9mouse.jpg "Anti-Bad (phospho S112) antibody [BADS112-B9] (ab278558)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [BADS112-B9] to Bad (phospho S112)
- Suitable for: Flow Cyt
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-Bad (phospho S112) antibody [BADS112-B9]
See all Bad primary antibodies -
Description
Rabbit monoclonal [BADS112-B9] to Bad (phospho S112) -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt Human
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Immunogen
Synthetic peptide within Human Bad (phospho S112). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q92934 -
Positive control
- Flow cyt: U937 cells (treated with Calyculin A); C6 cells (treated with pervanadate); NIH/3T3 cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituents: 49.88% PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA -
Concentration information loading... -
Purity
Protein A/G purified -
Clonality
Monoclonal -
Clone number
BADS112-B9 -
Isotype
IgG -
Light chain type
lambda -
Research areas
Images
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Flow Cytometry - Anti-Bad (phospho S112) antibody [BADS112-B9] (ab278558)ab278558 recognizes basal phosphorylation levels in mouse cells. Flow cytometric analysis of NIH3T3 cells secondary antibody only (blue) or 0.1 µg/mL of isotype control (orange) or of ab278558 (green).
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![Flow Cytometry - Anti-Bad (phospho S112) antibody [BADS112-B9] (ab278558)](https://www.abcam.com/ps/products/278/ab278558/Images/ab278558-3-bads112b9pepblock.jpg)
Flow Cytometry - Anti-Bad (phospho S112) antibody [BADS112-B9] (ab278558)Peptide blocking flow cytometric analysis of U937 cells secondary antibody only negative control (blue) or Calyculin A-treated (green) or CalA and blocked with phospho peptide (gold) or CalA and blocked with non-phospho peptide (purple) using ab278558 at 0.1 µg/mL. -
![Flow Cytometry - Anti-Bad (phospho S112) antibody [BADS112-B9] (ab278558)](https://www.abcam.com/ps/products/278/ab278558/Images/ab278558-2-bads112b9flow.jpg)
Flow Cytometry - Anti-Bad (phospho S112) antibody [BADS112-B9] (ab278558)Flow cytometric analysis of U937 cells secondary antibody only negative control (blue) or untreated (red) or treated with Calyculin A (green) using ab278558 at 0.01µg/mL.
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![Flow Cytometry - Anti-Bad (phospho S112) antibody [BADS112-B9] (ab278558)](https://www.abcam.com/ps/products/278/ab278558/Images/ab278558-5-ab278558comparisonflow.jpg)
Flow Cytometry - Anti-Bad (phospho S112) antibody [BADS112-B9] (ab278558)Flow cytometric analysis of C6 cells secondary antibody only negative control (blue), or treated with imatinib (red) or with pervanadate (green) using ab278558 at 0.08µg/mL.
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![Anti-Bad (phospho S112) antibody [BADS112-B9] (ab278558)](https://www.abcam.com/ps/products/278/ab278558/Images/ab278558-1-benefits-of-recombinant-antibodies.png)
Anti-Bad (phospho S112) antibody [BADS112-B9] (ab278558)