Recombinant Human EGF protein (ab55483)
Key features and details
- Expression system: Pichia pastoris
- Purity: > 95% SDS-PAGE
- Suitable for: WB, SDS-PAGE, Functional Studies
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Product name
Recombinant Human EGF protein
See all EGF proteins and peptides -
Purity
> 95 % SDS-PAGE.
ab55483 was purified by chromatographic techniques. Purity tested by SDS PAGE and RP-HPLC. -
Expression system
Pichia pastoris -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
The sequence of the first five N-terminal amino acids was determined and was found to be Asn-Ser-Asp-Ser-Glu, which agrees with the sequence of native human EGF. N-terminal methionine has been completely removed enzymatically. -
Predicted molecular weight
6 kDa -
Amino acids
971 to 1023
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Preparation and Storage
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Alternative names
- Beta urogastrone
- beta-urogastrone
- EGF
see all -
Function
EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Can induce neurite outgrowth in motoneurons of the pond snail Lymnaea stagnalis in vitro (PubMed:10964941). -
Tissue specificity
Expressed in kidney, salivary gland, cerebrum and prostate. -
Involvement in disease
Hypomagnesemia 4 -
Sequence similarities
Contains 9 EGF-like domains.
Contains 9 LDL-receptor class B repeats. -
Post-translational
modificationsO-glycosylated with core 1-like and core 2-like glycans. It is uncertain if Ser-954 or Thr-955 is O-glycosylated. The modification here shows glycan heterogeneity: HexHexNAc (major) and Hex2HexNAc2 (minor). -
Cellular localization
Membrane. - Information by UniProt
Images
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Western blot - Recombinant Human EGF protein (ab55483)
