Mouse Epiregulin ELISA Kit (EREG) (ab100682)
Key features and details
- Sensitivity: 10 pg/ml
- Range: 8.23 pg/ml - 6000 pg/ml
- Sample type: Cell culture supernatant, Plasma
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse Epiregulin ELISA Kit (EREG)
See all Epiregulin kits -
Detection method
Colorimetric -
Sample type
Cell culture supernatant, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
Range
8.23 pg/ml - 6000 pg/mlRecovery
83 %
Sample specific recovery Sample type Average % Range Cell culture supernatant 102.6 94% - 110% Plasma 73.94 67% - 90% Assay duration
Multiple steps standard assaySpecies reactivity
Reacts with: Mouse
Does not react with: HumanProduct overview
Abcam’s Epiregulin Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse Epiregulin in plasma and cell culture supernatants.
This assay employs an antibody specific for Mouse Epiregulin coated on a 96- well plate. Standards and samples are pipetted into the wells and Epiregulin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse Epiregulin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Epiregulin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
We have not been able to detect endogenous Mouse Epiregulin in normal serum with ab100682, only in serum spiked with Mouse Epiregulin.
Platform
MicroplateProperties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 300X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated anti-Mouse Epiregulin 2 vials Epiregulin Microplate (12 x 8 wells) 1 unit Recombinant Mouse Epiregulin Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Relevance
Epiregulin is a member of the EGF-related growth factor family. It is a mitogen and has numerous roles including as an insulinotropic factor in pancreatic beta cell lines. It may also be involved in metastasis. -
Alternative names
- EPR
- ER
- EREG
- Proepiregulin
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Database links
- SwissProt: Q61521 Mouse
Properties
-
Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 300X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated anti-Mouse Epiregulin 2 vials Epiregulin Microplate (12 x 8 wells) 1 unit Recombinant Mouse Epiregulin Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
-
Relevance
Epiregulin is a member of the EGF-related growth factor family. It is a mitogen and has numerous roles including as an insulinotropic factor in pancreatic beta cell lines. It may also be involved in metastasis. -
Alternative names
- EPR
- ER
- EREG
- Proepiregulin
-
Database links
- SwissProt: Q61521 Mouse
Images
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Typical Standard Curve
Representative Standard Curve using ab100682
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Typical Standard CurveRepresentative Standard Curve using ab100682