Human Amphiregulin ELISA Kit (ab222504)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 2.4 pg/ml
- Range: 18 pg/ml - 600 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Amphiregulin ELISA Kit
See all Amphiregulin kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Amphiregulin 3 1.8% Inter-assay Sample n Mean SD CV% Amphiregulin 5 4.1% -
Sample type
Cell culture supernatant, Serum, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
2.4 pg/ml -
Range
18 pg/ml - 600 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 106 84% - 117% Cell culture media 108 106% - 110% EDTA Plasma 98 91% - 104% Cit plasma 103 93% - 111% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human Amphiregulin ELISA Kit (ab222504) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Amphiregulin protein in cell culture supernatant, cit plasma, edta plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Amphiregulin with 2.4 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Amphiregulin is a member of the epidermal growth factor family encoded by the AREG gene. Amphiregulin is expressed as a 252- amino acid transmembrane glycoprotein, which contains an 81-amino acid N-terminal pro-peptide, a heparin binding domain, a EGF like domain, a 23- amino acid transmembrane domain, and a 31- amino acid cytoplasmic domain. The amphiregulin assay utilizes antibodies raised against the 101-187 amino acid region of the amphiregulin protein. Amphiregulin is an autocrine growth factor, a mitogen for several cell types, and is essential for mammary ductal development.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human Amphiregulin Capture Antibody 1 x 600µl 10X Human Amphiregulin Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml Human Amphiregulin Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Sample Diluent NS (ab193972) 1 x 50ml Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Bifunctional growth-modulating glycoprotein. Inhibits growth of several human carcinoma cells in culture and stimulates proliferation of human fibroblasts and certain other tumor cells. -
Sequence similarities
Belongs to the amphiregulin family.
Contains 1 EGF-like domain. -
Cellular localization
Membrane. - Information by UniProt
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Alternative names
- A REG
- Amphiregulin
- Amphiregulin B
see all -
Database links
- Entrez Gene: 374 Human
- Entrez Gene: 727738 Human
- Omim: 104640 Human
- SwissProt: P15514 Human
- Unigene: 270833 Human
- Unigene: 645475 Human
Images
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Other - Human Amphiregulin ELISA Kit (ab222504)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human Amphiregulin standard curve in Sample Diluent NS.The Amphiregulin standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of spiked Amphiregulin in human serum, plasma and cell culture supernatant samples.The concentrations of Amphiregulin were measured in duplicates, interpolated from the Amphiregulin standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (EDTA) 50%, plasma (citrate) 50%, RPMI media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
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Interpolated concentrations of native Amphiregulin in human MCF7 cell culture supernatant.Human MCF7s were stimulated with 2% PHA for 3 days before the supernatant was collected and assayed. The concentrations of Amphiregulin were measured in duplicate and interpolated from the Amphiregulin standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Amphiregulin concentration was determined to be 23.7 ng/mL in stimulated and 16 ng/mL in unstimulated MCF7 cell culture supernatant.
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Sandwich ELISA - Human Amphiregulin ELISA Kit (ab222504)To learn more about the advantages of recombinant antibodies see here.
