Human Epiregulin ELISA Kit (EREG) (ab277077)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 5.803 pg/ml
- Range: 6.25 pg/ml - 400 pg/ml
- Sample type: Cell culture supernatant, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Epiregulin ELISA Kit (EREG)
See all Epiregulin kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 7.3% Inter-assay Sample n Mean SD CV% Serum 3 8.1% -
Sample type
Cell culture supernatant, Serum -
Assay type
Sandwich (quantitative) -
Sensitivity
5.803 pg/ml -
Range
6.25 pg/ml - 400 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 102 95% - 108% Serum 83 80% - 86% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human Epiregulin ELISA kit (ab277077) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human Epiregulin protein in human serum and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Epiregulin with 5.8 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Human Epiregulin is a secreted peptide hormone encoded by the gene EREG. Epiregulin is a member of the epidermal growth factor family. Epiregulin is expressed as a type I membrane pro-form, proepiregulin, and epiregulin is catalytic cleaved to a 46- amino acid soluble form by disintegrin and metalloproteinase (ADAM). Epiregulin functions as a ligand of epidermal growth factor receptor (EGFR)and most members of the ERBB (v-erb-b2 oncogene homolog) family of tyrosine-kinase receptors. Epiregulin contributes to inflammation, wound healing, tissue repair, and oocyte maturation.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human Epiregulin (EREG) Capture Antibody 1 x 600µl 10X Human Epiregulin (EREG) Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BI 1 x 6ml Human Epiregulin (EREG) Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Relevance
Epiregulin is a member of the EGF-related growth factor family. It is a mitogen and has numerous roles including as an insulinotropic factor in pancreatic beta cell lines. It may also be involved in metastasis. -
Alternative names
- EPR
- ER
- EREG
- Proepiregulin
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Database links
- SwissProt: O14944 Human
Images
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Serum from eight individual healthy Epiregulin female donors was measured in duplicate.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Epiregulin concentration was determined to be 23.5 pg/mL. Seven samples tested below the detection limit on the assay.
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SimpleStep ELISA Protocol DiagramSimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human Epiregulin standard curve in Sample Diluent NS.The Epiregulin standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native Epiregulin in human U-87 cell supernatant.The concentrations of Epiregulin were measured in duplicates, interpolated from the target standard curves and corrected for sample dilution. Undiluted samples are as follows: U-87 cell supernatant 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean target concentration was determined to be 244.1 pg/mL in U-87 cell supernatant.
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Sandwich ELISA - Human Epiregulin ELISA Kit (EREG)To learn more about the advantages of recombinant antibodies see here.
