Rabbit Leptin ELISA Kit (ab273241)
Key features and details
- Sensitivity: 0.2 ng/ml
- Range: 0.205 ng/ml - 50 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Rabbit
Overview
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Product name
Rabbit Leptin ELISA Kit
See all Leptin kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
0.2 ng/ml -
Range
0.205 ng/ml - 50 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 101.2 83% - 121% Plasma 88.68 67% - 109% Cell culture media 91.98 73% - 106% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Rabbit -
Product overview
Rabbit Leptin ELISA Kit (ab273241) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rabbit Leptin in serum (Rabbit Leptin concentration is low in normal serum and plasma samples, and may not be detectable in this assay), plasma and cell culture supernatants.
This assay employs an antibody specific for Rabbit Leptin coated on a 96-well plate. Standards and samples are pipetted into the wells and Leptin present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Rabbit Leptin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Leptin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml Anti-Rabbit Leptin coated Microplate (12 x 8 wells) 1 unit Assay Diluent C 1 x 30ml Biotinylated Anti-Rabbit Leptin Antibody 2 vials Rabbit Leptin Standard (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB Substrate Solution 1 x 12ml -
Research areas
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Function
May function as part of a signaling pathway that acts to regulate the size of the body fat depot. An increase in the level of LEP may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass. -
Involvement in disease
Defects in LEP may be a cause of obesity (OBESITY) [MIM:601665]. It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat. -
Sequence similarities
Belongs to the leptin family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- FLJ94114
- LEP
- LEP_HUMAN
see all
Images
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Rabbit Leptin ELISA Kit standard curve
This standard curve is for demonstration only. A standard curve must be run with each assay.