Recombinant human DPP4 protein (ab79138)
Key features and details
- Expression system: Baculovirus infected BTI-TN-5B1-4 cells
- Purity: > 95% SDS-PAGE
- Active: Yes
- Suitable for: WB, SDS-PAGE
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Product name
Recombinant human DPP4 protein
See all DPP4 proteins and peptides -
Biological activity
Biological activity: > 20 unit/mg. One unit will hydrolyze 1 µmole of p-nitroaniline per minute at pH8.0 at 37C using 1mM of Gly-Pro p-nitroanilde as a substrate. -
Purity
> 95 % SDS-PAGE.
ab79138 is purified using conventional chromatography techniques. -
Expression system
Baculovirus infected BTI-TN-5B1-4 cells -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
SRKTYTLTDYLK NTYRLKLYSL RWISDHEYLY KQENNILVFN AEYGNSSVFL ENSTFDEFGH SINDYSISPD GQFILLEYNY VKQWRHSYTA SYDIYDLNKR QLITEERIPN NTQWVTWSPV GHKLAYVWNN DIYVKIEPNL PSYRITWTGK EDIIYNGITD WVYEEEVFSA YSALWWSPNG TFLAYAQFND TEVPLIEYSF YSDESLQYPK TVRVPYPKAG AVNPTVKFFV VNTDSLSSVT NATSIQITAP ASMLIGDHYL CDVTWATQER ISLQWLRRIQ NYSVMDICDY DESSGRWNCL VARQHIEMST TGWVGRFRPS EPHFTLDGNS FYKIISNEEG YRHICYFQID KKDCTFITKG TWEVIGIEAL TSDYLYYISN EYKGMPGGRN LYKIQLSDYT KVTCLSCELN PERCQYYSVS FSKEAKYYQL RCSGPGLPLY TLHSSVNDKG LRVLEDNSAL DKMLQNVQMP SKKLDFIILN ETKFWYQMIL PPHFDKSKKY PLLLDVYAGP CSQKADTVFR LNWATYLAST ENIIVASFDG RGSGYQGDKI MHAINRRLGT FEVEDQIEAA RQFSKMGFVD NKRIAIWGWS YGGYVTSMVL GSGSGVFKCG IAVAPVSRWE YYDSVYTERY MGLPTPEDNL DHYRNSTVMS RAENFKQVEY LLIHGTADDN VHFQQSAQIS KALVDVGVDF QAMWYTDEDH GIASSTAHQH IYTHMSHFIK QCFSLP SGRLVPRGSHHHHHH -
Amino acids
39 to 766
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Preparation and Storage
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Stability and Storage
Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.316% Tris HCl, 0.0292% EDTA, 10% Glycerol (glycerin, glycerine), 0.58% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
Images
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SDS-PAGE - Recombinant human DPP4 protein (ab79138)10% SDS-PAGE showing ab79138 at approximately 90kDa (2µg).
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Functional Studies - Recombinant human DPP4 protein (ab79138)Optical density was measured at 405 nm after incubating enzyme solution with 1mM of p-nitroanilid as a substrate. -
Western blot - Recombinant human DPP4 protein (ab79138)All lanes : Anti-DPP4 antibody (ab86806) at 1 µg/ml
Lane 1 :Recombinant human DPP4 protein (ab79138) at 0.1 µg
Lane 2 :Recombinant human DPP4 protein (ab79138) at 0.01 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 10 seconds
