Recombinant human DNase I protein (Active) (ab275555)
Key features and details
- Expression system: Escherichia coli
- Active: Yes
- Tags: His tag N-Terminus
- Suitable for: SDS-PAGE, WB, Functional Studies
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Product name
Recombinant human DNase I protein (Active)
See all DNase I proteins and peptides -
Purity
>90% by SDS-PAGE. -
Expression system
Escherichia coli -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
GA VSLKIAAFNI QTFGETKMSN ATLVSYIVQI LSRYDIALVQ EVRDSHLTAV GKLLDNLNQD APDTYHYVVS EPLGRNSYKE RYLFVYRPDQ VSAVDSYYYD DGCEPCGNDT FNREPAIVRF FSRFTEVREF AIVPLHAAPG DAVAEIDALY DVYLDVQEKW GLEDVMLMGD FNAGCSYVRP SQWSSIRLWT SPTFQWLIPD SADTTATPTH CAYDRIVVAG MLLRGAVVPD SALPFNFQA -
Predicted molecular weight
31 kDa -
Amino acids
19 to 259 -
Tags
His tag N-Terminus
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Preparation and Storage
- Deoxyribonuclease 1
- Deoxyribonuclease I
- Deoxyribonuclease-1
Images
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Functional Studies - Recombinant human DNase I protein (Active) (ab275555)
The binding activity of DNase I and ACTb.
DNase I were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to ACTb-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-DNase I pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately.
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Western blot - Recombinant human DNase I protein (Active) (ab275555)Rabbit anti-human DNase IA + Recombinant human DNase I protein
Predicted band size: 31 kDa
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SDS-PAGE - Recombinant human DNase I protein (Active) (ab275555)SDS-PAGE - Recombinant human DNase I protein (Active) (ab275555).