Astana Biomed Group, an authorized Abcam distributor in Central Asia

Human Cardiotoxicity FirePlex®-HT Panel 1 (ab252375)

Overview

  • Product name

    Human Cardiotoxicity FirePlex®-HT Panel 1

  • Detection method

    Fluorescent

  • Sample type

    Cell culture supernatant, Serum, Plasma, Hep Plasma, EDTA Plasma, Cit plasma

  • Assay type

    Multiplex

  • Species reactivity

    Reacts with: Human

  • Product overview

    Human Cardiotoxicity FirePlex®-HT Panel 1 (ab252375) uses FirePlex® particle technology to quantify ICAM1, Cardiac Troponin I, FABP3, NT-proBNP, and BNP from 6.25 µl of sample input in 384-well plate format.


    Assays are validated in serum, plasma, and cell culture supernatant, and can be run with 175 samples in duplicate per 384-well plate.


    The two-step workflow and no-wash assay format limit hands-on time and are amenable to automation, thus making FirePlex-HT ideally suited for high-throughput screening studies. Assay readout is conducted on high-content imagers with data analysis using the FirePlex® Analysis Workbench software.


    Users should note that assay performance data supplied in the accompanying Product Datasheet is a representative dataset, and is not lot-specific. For lot-specific assay performance data, please refer to the product's certificate of analysis, which can be found here.


    FirePlex® is a registered trade mark in certain territories (including Europe and the United States) and is an unregistered trade mark elsewhere.

  • Notes

    This product is currently only supported for customers in North America and Europe, if you are outside these areas please contact us.

    The following table provides the sensitivity, dynamic range, and CVs for Human Cardiotoxicity FirePlex®-HT Panel 1 *.

    Target 

    Sensitivity/LOD

    (pg/ml) 

    Dynamic Range

    (pg/ml) 

     Inter-well CV

    (Std curve)
    BNP  8.71 13.7 - 3,333  13.3%
    Cardiac Troponin I∇  92.60 123 - 30,000 9.2% 
    FABP3∇  6.42 13.7 - 1,111 8.6%
    ICAM1  26.79 41.1 - 30,000 10.7%
    NT-proBNP  15.53 41.1 - 3,333 11.7%

    *representative dataset, not lot-specific. For lot-specific assay performance data, please refer to the product's certificate of analysis, which can be found here.

    ∇The bovine and human proteins for these analytes exhibit a >90% sequence homology, thus some bovine serum cross-reactivity may be observed in cell culture supernatant (CCS) samples. To address this, we strongly recommend that users include a cell culture media-only control in experiments using CCS samples.

  • Platform

    Multiplex

Properties

Images

  • Assay Workflow
    Assay Workflow
    Assay Workflow

     

  • Standard Curve Plot
    Standard Curve Plot
    Standard Curve

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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