Astana Biomed Group, an authorized Abcam distributor in Central Asia

Recombinant Human DCK protein (ab173027)

Key features and details

  • Expression system: Escherichia coli
  • Purity: > 95% SDS-PAGE
  • Endotoxin level:
  • Tags: His tag N-Terminus
  • Suitable for: SDS-PAGE, HPLC

Description

  • Product name

    Recombinant Human DCK protein
    See all DCK proteins and peptides

  • Purity

    > 95 % SDS-PAGE.
    Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE.

  • Endotoxin level

  • Expression system

    Escherichia coli

  • Accession

    P27707

  • Protein length

    Full length protein

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Sequence

      MGSSHHHHHHSSGLVPRGSHMASMTGGQQMGRGSMATPPKRSCPSFSASS EGTRIKKISIEGNIAAGKSTFVNILKQLCEDWEVVPEPVARWCNVQSTQD EFEELTMSQKNGGNVLQMMYEKPERWSFTF QTYACLSRIRAQLASLNGKLKDAEKPVLFFERSVYSDRYIFASNLYESEC MNETEWTIYQDWHDWMNNQFGQSLELDGIIYLQATPETCLHRIYLRGRNE EQGIPLEYLEKLHYKHESWLLHRTLKTNFDYLQEVPILTLDVNEDFKDKY ESLVEKVKEFLSTL

    • Predicted molecular weight

      34 kDa including tags

    • Amino acids

      1 to 260

    • Tags

      His tag N-Terminus

    • Specifications

    Our Abpromise guarantee covers the use of ab173027 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      SDS-PAGE

      HPLC

    • Form

      Liquid
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped on Dry Ice. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

      pH: 7.50
      Constituent: 0.24% Tris

      Supplied as a 0.2 µM filtered solution.

    General Info

    • Alternative names

      • dCK
      • DCK protein
      • DCK_HUMAN
      • Deoxycytidine kinase
      • Deoxynucleoside kinase
      • EC 2.7.1
      • EC 2.7.1.74
      • MGC117410
      • MGC138632
      • OTTHUMP00000219118
      • OTTHUMP00000219119
      see all

    • Function

      Required for the phosphorylation of the deoxyribonucleosides deoxycytidine (dC), deoxyguanosine (dG) and deoxyadenosine (dA). Has broad substrate specificity, and does not display selectivity based on the chirality of the substrate. It is also an essential enzyme for the phosphorylation of numerous nucleoside analogs widely employed as antiviral and chemotherapeutic agents.

    • Sequence similarities

      Belongs to the DCK/DGK family.

    • Post-translational
      modifications

      Phosphorylated upon DNA damage, probably by ATM or ATR.

    • Cellular localization

      Nucleus.
    • Information by UniProt

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab173027? Please let us know so that we can cite the reference in this datasheet.

ab173027 has not yet been referenced specifically in any publications.

Preparation and Storage

  • Stability and Storage

    Shipped on Dry Ice. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    pH: 7.50
    Constituent: 0.24% Tris

    Supplied as a 0.2 µM filtered solution.

Images

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