Recombinant Human DC-SIGNR protein (Fc Chimera) (ab83920)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Suitable for: SDS-PAGE
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Product name
Recombinant Human DC-SIGNR protein (Fc Chimera)
See all DC-SIGNR proteins and peptides -
Purity
> 95 % SDS-PAGE. -
Expression system
HEK 293 cells -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
Theoretical Sequence: HGTELPSPPSKLQVSKVPSSLSQEQSEQDAIYQNLTQLKAAVGELSEK SKLQEIYQELTQLKAAVGELPEKSKLQEIYQELTRLKAAVGELPEKSKLQ EIYQELTRLKAAVGELPEKSKLQEIYQELTRLKAAVGELPEKSKLQEIYQ ELTELKAAVGELPEKSKLQEIYQELTQLKAAVGELPDQSKQQQIYQELTD LKTAFERLCRHCPKDWTFFQGNCYFMSNSQRNWHDSVTACQEVRAQLVVI KTAEEQNFLQLQTSRSNRFSWMGLSDLNQEGTWQWVDGSPLSPSFQRYWN SGEPNNSGNEDCAEFSGSGWNDNRCDVDNYWICKKPAACFRDGIPKVDKK VEPKSCDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVV DVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWL NGKEYKCRVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQVS LTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDK SRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK -
Additional sequence information
Fused with the Fc region of Human IgG1 at the C-terminus.
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Preparation and Storage
- C type lectin domain family 4, member M
- C-type lectin domain family 4 member M
- CD209 antigen like
Images
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SDS-PAGE - Recombinant Human DC-SIGNR protein (Fc Chimera) (ab83920)Lane 1 – MW markers; Lane 2 – ab83920; Lane 3 – ab83920 treated with PNGase F to remove potential N-linked glycans. 10 μg protein loaded per lane. Deep Purple stained.
Drop in MW after treatment with PNGase F indicates presence of N-linked glycans. Band in lane 3 at 35 kDa is PNGase F protein. -
SDS-PAGE - Recombinant Human DC-SIGNR protein (Fc Chimera) (ab83920)A sample of ab83920 without carrier protein was reduced and alkylated. 40 μg protein was loaded, focused on a 3-10 IPG strip then run on a 4-20% Tris-HCl 2D gel. Spot train (Deep Purple™ stained) indicates presence of multiple glycoforms of ab83920. Spots within the spot train were cut from the gel and identified by protein mass fingerprinting as DC-SIGNR (Fc Chimera).
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Functional Studies - Recombinant Human DC-SIGNR protein (Fc Chimera) (ab83920)Post-translational modifications result in protein heterogeneity. The densitometry scan demonstrates the purified human cell expressed protein exists in multiple glycoforms, which differ according to their level of post-translational modification.
The triangle indicates theoretical pI and MW.