Astana Biomed Group, an authorized Abcam distributor in Central Asia

Recombinant Human Cysteine Dioxygenase CDO1 protein (ab153778)

Key features and details

  • Expression system: Escherichia coli
  • Purity: > 95% SDS-PAGE
  • Endotoxin level:
  • Tags: His tag N-Terminus
  • Suitable for: SDS-PAGE, HPLC

Description

  • Product name

    Recombinant Human Cysteine Dioxygenase CDO1 protein

  • Purity

    > 95 % SDS-PAGE.
    ab153778 is greater than 95% pure, as determined by SEC-HPLC and reducing SDS-PAGE. It is lyophilized from an 0.2 µM filtered solution.

  • Endotoxin level

  • Expression system

    Escherichia coli

  • Accession

    Q16878

  • Protein length

    Full length protein

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Sequence

      MEQTEVLKPRTLADLIRILHQLFAGDEVNVEEVQAIMEAYESDPTEWAMY AKFDQYRYTRNLVDQGNGKFNLMILCWGEGHGSSIHDHTNSHCFLKMLQG NLKETLFAWPDKKSNEMVKKSERVLRENQCAYINDSIGLHRVENISHTEP AVSLHLYSPPFDTCHAFDQRTGHKNKVTMTFHSKFGIRTPNATSGSLENN

    • Predicted molecular weight

      23 kDa

    • Amino acids

      1 to 200

    • Tags

      His tag N-Terminus

    • Specifications

    Our Abpromise guarantee covers the use of ab153778 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      SDS-PAGE

      HPLC

    • Form

      Liquid

    • Additional notes

      Previously labelled as Cysteine Dioxygenase Type 1.

    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

      pH: 8.00
      Constituents: 0.32% Tris HCl, 10% Glycerol (glycerin, glycerine), 0.88% Sodium chloride

    General Info

    • Alternative names

      • CDO
      • CDO 1
      • CDO I
      • CDO-I
      • Cdo1
      • CDO1_HUMAN
      • CDOI
      • Cysteine dioxygenase type 1
      • Cysteine dioxygenase type I
      • Cytosolic cysteine dioxygenase
      see all

    • Function

      Initiates several important metabolic pathways related to pyruvate and several sulfurate compounds including sulfate, hypotaurine and taurine. Critical regulator of cellular cysteine concentrations. Has an important role in maintaining the hepatic concentation of intracellular free cysteine within a proper narrow range.

    • Tissue specificity

      Highly expressed in liver and placenta. Low expression in heart, brain and pancreas. Also detected in hepatoblastoma HepG2 cells.

    • Pathway

      Organosulfur biosynthesis; taurine biosynthesis; hypotaurine from L-cysteine: step 1/2.

    • Sequence similarities

      Belongs to the cysteine dioxygenase family.

    • Post-translational
      modifications

      The thioether cross-link between Cys-93 and Tyr-157 plays a structural role through stabilizing the Fe(2+) ion, and prevents the production of highly damaging free hydroxyl radicals by holding the oxygen radical via hydroxyl hydrogen.
    • Information by UniProt

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab153778? Please let us know so that we can cite the reference in this datasheet.

ab153778 has not yet been referenced specifically in any publications.

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 8.00
    Constituents: 0.32% Tris HCl, 10% Glycerol (glycerin, glycerine), 0.88% Sodium chloride

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