Recombinant Human Claudin 4 protein (ab124320)
Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Tags: His tag N-Terminus
- Suitable for: WB, ELISA, SDS-PAGE
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Product name
Recombinant Human Claudin 4 protein
See all Claudin 4 proteins and peptides -
Purity
> 90 % SDS-PAGE.
ab124320 was purifed using Ni-NTA chromatography. The product is filtered (0.4 µm) but not sterile, hence needs to be filtered by an appropriate sterile filter before using in cell culture. -
Expression system
Escherichia coli -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MKHHHHHHAS MWRVTAFIGS NIVTSQTIWE GLWMNCVVQS TGQMQCKVYD SLLALPQDLQ AARLEVLFQG PTAHNIIQDF YNPLVASGQK REM -
Predicted molecular weight
11 kDa -
Tags
His tag N-Terminus
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Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle. Please see notes section.
Constituent: 99% Sodium acetate
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ReconstitutionAdd 0.1 M Acetate buffer pH 4.0 to prepare a working stock solution of 0.5 mg/mL and let the lyophilized pellet dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10 µg/mL. In higher concentrations the solubility of this antigen is limited.
Images
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14% SDS-PAGE separation of Human Claudin 4 recombinant fusion protein (ab124309)
Lane 1: reduced and heated sample (5 µg)
Lane 2: non-reduced and non-heated sample (5 µg)