Human IGFBP1 ELISA Kit (ab233618)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 11.6 pg/ml
- Range: 17.2 pg/ml - 1100 pg/ml
- Sample type: Cell culture extracts, Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Milk, Serum, Tissue Homogenate, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human IGFBP1 ELISA Kit
See all IGFBP1 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Supernatant 5 5.1% Inter-assay Sample n Mean SD CV% Supernatant 3 6.3% -
Sample type
Cell culture supernatant, Milk, Urine, Serum, Cell culture extracts, Hep Plasma, EDTA Plasma, Cit plasma, Tissue Homogenate -
Assay type
Sandwich (quantitative) -
Sensitivity
11.6 pg/ml -
Range
17.2 pg/ml - 1100 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Milk 101 90% - 119% Urine 97 91% - 109% Serum 99 94% - 104% Cell culture extracts 100 89% - 112% Hep Plasma 102 97% - 108% EDTA Plasma 103 97% - 111% Cit plasma 104 101% - 108% Tissue Homogenate 101 93% - 108% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human IGFBP1 ELISA Kit (ab233618) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IGFBP1 protein in cit plasma, edta plasma, hep plasma, milk, serum, tissue homogenate, urine, cell culture supernatant, and cell culture extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human IGFBP1 with 11.6 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human IGFBP1 Capture Antibody 1 x 600µl 10X Human IGFBP1 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 4BI 1 x 6ml Human IGFBP1 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Promotes cell migration. -
Sequence similarities
Contains 1 IGFBP N-terminal domain.
Contains 1 thyroglobulin type-1 domain. -
Post-translational
modificationsPhosphorylated; probably by casein kinase II. Alters the affinity of the protein for IGFs. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- AFBP
- Alpha pregnancy associated endometrial globulin
- Amniotic fluid binding protein
see all -
Database links
- Entrez Gene: 3484 Human
- Omim: 146730 Human
- SwissProt: P08833 Human
- Unigene: 642938 Human
Images
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Other - Human IGFBP1 ELISA Kit (ab233618)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Human IGFBP1 Competitor standard curve comparisonStandard curve comparison between Human IGFBP1 SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.
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Example of human IGFBP1 standard curve in Sample Diluent NS.The IGFBP1 standard curve was prepared as described in Section 10.
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Example of human IGFBP1 standard curve in 1X Cell Extraction Buffer PTR. -
Interpolated concentrations of native IGFBP1 in human serum and plasma samples.The concentrations of IGFBP1 were measured in duplicates, interpolated from the IGFBP1 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 2.5%, plasma (citrate) 5%, plasma (heparin) 5%, and plasma (EDTA) 2.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IGFBP1 concentration was determined to be 22 ng/mL in serum, 16 ng/mL in plasma (citrate), 21 ng/ml in plasma (heparin), and 37 ng/ml in plasma (EDTA).
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Interpolated concentrations of native IGFBP1 in human milk and cell culture supernatant samples.The concentrations of IGFBP1 were measured in duplicates, interpolated from the IGFBP1 standard curves and corrected for sample dilution. Undiluted samples are as follows: milk 5%, A549 supernatant 100%, and HepG2 supernatant 0.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IGFBP1 concentration was determined to be 12 ng/ml in milk, 0.5 ng/ml in A549 supernatant, and 172 ng/mL in HepG2 supernatant.
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. Interpolated concentrations of native IGFBP1 in human HepG2, U-87 MG, and liver homogenate extract samples based on a 4 µg/mL, 500 µg/ml, and 500 µg/mL extract load respectivelyThe concentrations of IGFBP1 were measured in duplicate and interpolated from the IGFBP1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IGFBP1 concentration was determined to be 580 pg/mL in HepG2 cell extract, 457 pg/ml in U-87 MG cell extract, and 739 pg/mL in liver tissue homogenate extract samples.
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Serum from ten individual healthy human male donors was measured in duplicate.Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IGFBP1 concentration was determined to be 29.1 ng/mL with a range of 1.5 – 117.3 ng/mL.
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Sandwich ELISA - Human IGFBP1 ELISA Kit (ab233618)To learn more about the advantages of recombinant antibodies see here.
