Antibodies, Proteins, Kits and Reagents for Life Science

Key features and details

Expression system: Mammalian
Purity: > 95% SDS-PAGE
Endotoxin level:
Suitable for: SDS-PAGE, HPLC

Product name

Recombinant Human CD99 protein
See all CD99 proteins and peptides
Purity

> 95 % SDS-PAGE.
ab186093 is greater than 95% pure, as determined by SEC-HPLC and reducing SDS-PAGE.
Endotoxin level
Expression system

Mammalian
Accession

P14209
Protein length

Protein fragment
Animal free

No
Nature

Recombinant
- Species
  
  Human
- Sequence
  
  DGGFDLSDALPDNENKKPTAIPKKPSAGDDFDLGDAVVDGENDDPRPPNP PKPMPNPNPNHPSSSGSFSDADLADGVSGGEGKGGSDGGGSHRKEGEEAD VDDIEGRMDEPKSCDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRT PEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVL TVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRE EMTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFL YSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK
- Predicted molecular weight
  
  37 kDa including tags
- Amino acids
  
  23 to 122
- Additional sequence information
  
  FC tag at C terminus. Extracellular domain.

Related Products
- Anti-CD99 antibody [EPR3096] (ab108297)

Our Abpromise guarantee covers the use of ab186093 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Applications

SDS-PAGE

HPLC
Form

Lyophilized
Concentration information loading...

Stability and Storage

Shipped at 4°C. The lyophilized protein is stable for a few weeks at room temperature. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

pH: 7.40
Constituent: 100% PBS

0.2 µM filtered solution
Reconstitution

Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 µg/ml. Dissolve the lyophilized protein in 3X PBS. Please aliquot the reconstituted solution

Alternative names
- 12E7
- Antigen identified by monoclonal 12E7, Y homolog
- Antigen identified by monoclonal antibodies 12E7, F21 and O13
- CD99
- CD99 antigen
- CD99 molecule
- CD99_HUMAN
- Cell surface antigen 12E7
- Cell surface antigen HBA 71
- Cell surface antigen O13
- E2 antigen
- HBA71
- MIC 2X
- MIC 2Y
- MIC2
- MIC2 (monoclonal antibody 12E7)
- MIC2X
- MIC2Y
- MSK5X
- Protein MIC2
- Surface antigen MIC2
- T cell surface glycoprotein E2
- T-cell surface glycoprotein E2
see all
Function

Involved in T-cell adhesion processes. It is involved in spontaneous rosette formation with erythrocytes.
Sequence similarities

Belongs to the CD99 family.
Post-translational
modifications

Extensively O-glycosylated.
Cellular localization

Membrane.
Target information above from: UniProt accession P14209 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheet

Publishing research using ab186093? Please let us know so that we can cite the reference in this datasheet.

ab186093 has not yet been referenced specifically in any publications.

Stability and Storage

Shipped at 4°C. The lyophilized protein is stable for a few weeks at room temperature. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

pH: 7.40
Constituent: 100% PBS

0.2 µM filtered solution
Reconstitution

Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 µg/ml. Dissolve the lyophilized protein in 3X PBS. Please aliquot the reconstituted solution