Human CD38 ELISA Kit (ab267801)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 7.44 pg/ml
- Range: 31.25 pg/ml - 2000 pg/ml
- Sample type: Cell culture supernatant, Cell Lysate, Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human CD38 ELISA Kit
See all CD38 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 5.2% Inter-assay Sample n Mean SD CV% Serum 3 7.9% -
Sample type
Cell culture supernatant, Serum, Cell Lysate, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
7.44 pg/ml -
Range
31.25 pg/ml - 2000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 107 98% - 117% Serum 85 82% - 88% Cell Lysate 87 75% - 94% Hep Plasma 88 85% - 90% EDTA Plasma 82 76% - 90% Cit plasma 91 90% - 93% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human CD38 ELISA Kit (ab267801) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CD38 protein in cell culture supernatant, cell lysate, edta plasma, hep plasma, serum, and cit plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human CD38 with 7.44 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
CD38, also known as ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1 (EC:3.2.2.6), is a single-pass type II membrane protein. CD38 catalyzes synthesis of cyclic ADP-ribose, a second messenger for glucose-induced insulin secretion. It also has a cyclic ADP-ribose hydrolase activity.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human CD38 Capture Antibody 1 x 600µl 10X Human CD38 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 5BI 1 x 6ml Human CD38 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Synthesizes cyclic ADP-ribose, a second messenger for glucose-induced insulin secretion. Also has cADPr hydrolase activity. Also moonlights as a receptor in cells of the immune system. -
Tissue specificity
Expressed at high levels in pancreas, liver, kidney, brain, testis, ovary, placenta, malignant lymphoma and neuroblastoma. -
Sequence similarities
Belongs to the ADP-ribosyl cyclase family. -
Developmental stage
Preferentially expressed at both early and late stages of the B and T-cell maturation. It is also detected on erythroid and myeloid progenitors in bone marrow, where the level of surface expression was shown to decrease during differentiation of blast-forming unit E to colony-forming unit E. -
Cellular localization
Membrane. - Information by UniProt
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Alternative names
- Acute lymphoblastic leukemia cells antigen CD38
- ADP ribosyl cyclase
- ADP ribosyl cyclase 1
see all -
Database links
- Entrez Gene: 952 Human
- Omim: 107270 Human
- SwissProt: P28907 Human
- Unigene: 479214 Human
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human CD38 standard curve in Sample Diluent NS.The CD38 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native CD38 in human Daudi cell culture supernatant sample.The concentrations of CD38 were measured in duplicates, interpolated from the CD38 standard curves and corrected for sample dilution. Undiluted samples are as follows: Daudi cell culture supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD38 concentration was determined to be 1,493 pg/mL in neat Daudi supernatant.
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Interpolated concentrations of native CD38 in human serum, plasma-citrate, plasma-EDTA and plasma-heparin samples.The concentrations of CD38 were measured in duplicates in neat samples and interpolated from the CD38 standard curves. The interpolated values are plotted (mean +/- SD, n=2). The mean CD38 concentration was determined to be 47.6 pg/mL in serum, 35.0 pg/mL in plasma-citrate, 51.4 pg/mL in plasma-EDTA and 39.5 pg/mL in plasma-heparin.
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Interpolated concentrations of native CD38 in human Raji and Daudi cells based on a 5 µg/mL extract load.The concentrations of CD38 were measured in duplicate and interpolated from the CD38 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD38 concentration was determined to be 1,268 pg/mL in Raji extract and 1,323 pg/mL in Daudi extract.
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Serum from ten individual healthy human female donors was measured in duplicate.Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD38 concentration was determined to be 51.8 pg/mL with a range of 36.6 – 82.2 pg/mL.
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Sandwich ELISA - Human CD38 ELISA Kit (ab267801)To learn more about the advantages of recombinant antibodies see here.
