Anti-CD74 antibody [LN-2] - BSA and Azide free (ab213104)
Key features and details
- Mouse monoclonal [LN-2] to CD74 - BSA and Azide free
- Suitable for: IHC-P, Flow Cyt (Intra)
- Knockout validated
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-CD74 antibody [LN-2] - BSA and Azide free
See all CD74 primary antibodies -
Description
Mouse monoclonal [LN-2] to CD74 - BSA and Azide free -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt (Intra) HumanIHC-P Human -
Immunogen
Tissue, cells or virus corresponding to Human CD74. (SU-DHL-4 lymphoma cells).
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Positive control
- Human tonsil and lymph node tissues; Daudi and Raji cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
ab213104 was purified from Bioreactor Concentrate by Protein A/G. -
Clonality
Monoclonal -
Clone number
LN-2 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human tonsil tissue labeling CD74 with ab213104 at 2 µg/ml.
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This data was developed using the same antibody clone in a different buffer formulation (ab9514)
Flow cytometry overlay histogram showing wild-type Raji (green line) and CD74 knockout Raji cells (ab273378) stained with ab9514 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS containing 10μg/ml human IgG and 10% normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab9514) (1x106 in 100μl at 1 μg/ml) for 30 min at 22°C.
The secondary antibody Goat anti-mouse IgG H&L (Alexa Fluor® 488, pre-adsorbed) (ab150117) was used at 1/2000 for 30 min at 22°C.
Isotype control antibody was mouse IgG1κ (ab170190) used at the same concentration and conditions as the primary antibody (wild-type Raji cells - black line; CD74 knockout Raji cells ab273378 - grey line). Unlabelled sample was also used as a control (this line is not shown for the purpose of simplicity).
Acquisition of >5000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter.
This antibody gave a positive signal in CD74 knockout Raji cells fixed with 4% formaldehyde (10 min) / permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.