Recombinant human CD3 epsilon protein (Active) (ab167755)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 90% SDS-PAGE
- Endotoxin level:
- Active: Yes
- Tags: His tag C-Terminus
- Suitable for: Functional Studies, SDS-PAGE
Preparation and Storage
-
Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. For long term storage it is recommended to add a carrier protein on reconstitution (0.1% HSA or BSA). Working aliquots stored with a carrier protein are stable for at least 3 months at -20°C to -80°C..
pH: 7.40
Constituents: PBS, 5% Trehalose
Lyophilized from 0.22 µm filtered solution.
5-10% trehalose is commonly used for freeze drying, and after reconstitution, the trehalose is mostly about 3-5%This product is an active protein and may elicit a biological response in vivo, handle with caution.
-
ReconstitutionReconstitute with sterile deionized water to a concentration of 600 µg/ml.
Images
-
ELISA - Recombinant human CD3 epsilon protein (ab167755)
Immobilized Mouse Anti-Human CD3 at 2 μg/mL (100 μL/well) binds ab167755.
Linear range: 0.4-6.25 ng/mL (QC tested).
-
Functional Studies - Recombinant human CD3 epsilon protein (ab167755)SP34-2 (Mouse IgG1) captured on CM5 chip via anti-mouse antibodies surface, binds ab167755 with an affinity constant of 0.97 nM , as determined in a SPR assay (Biacore T200) (Routinely tested).
-
SDS-PAGE - Recombinant human CD3 epsilon protein (ab167755)Reduced ab167755 on SDS-PAGE, stained overnight with Coomassie Blue.
Purity of protein >90%
The protein migrates as 15-16 kDa under reducing conditions.
