Recombinant human Cathepsin F protein (ab198436)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 90% SDS-PAGE
- Active: Yes
- Tags: His tag C-Terminus
- Suitable for: Functional Studies, SDS-PAGE
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Product name
Recombinant human Cathepsin F protein
See all Cathepsin F proteins and peptides -
Biological activity
≥ 0.9 pmole/min/µg. Enzyme activity was measured in a buffer containing 100 mM Sodium acetate (pH 5.5), 2 mM EDTA, 8 mM DTT, 0.05% Brij, and 20 µM substrate (Z-FRAMC), with 3.75-30 ng/µl Cathepsin F. Reaction was carried out at 30°C for 40-120 min, and fluorescence signal was measured at Ex360 nm/Em460 nm. Enzyme was preactivated in 50 mM sodium acetate pH 4.5, 5 mM DTT buffer for 90 min at RT prior to reaction.
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Purity
> 90 % SDS-PAGE.
Purity varies with Lot. -
Expression system
HEK 293 cells -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Predicted molecular weight
53 kDa including tags -
Amino acids
20 to 484 -
Tags
His tag C-Terminus -
Additional sequence information
Mature protein Cathepsin F; NM_003793. Also contains CD33 signal peptide (a.a. 1-16). MWt. is more than 58kDa after post-modification. Expressed in FreeStyle 293-F cells.
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Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.5
Constituents: 0.36% Sodium acetate, 0.78% Sodium chloride, 10% Glycerol (glycerin, glycerine)This product is an active protein and may elicit a biological response in vivo, handle with caution.
Images
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Functional Studies - Recombinant human Cathepsin F protein (ab198436)
Activity assay of ab198436.
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SDS-PAGE - Recombinant human Cathepsin F protein (ab198436)10% SDS-PAGE analysis of 3 µg ab198436 with Coomassie staining.
