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Anti-Actin Regulatory Protein CAPG/MCP antibody [EPR13194] (ab181092)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13194] to Actin Regulatory Protein CAPG/MCP
  • Suitable for: WB, IP
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-Actin Regulatory Protein CAPG/MCP antibody [EPR13194]
    See all Actin Regulatory Protein CAPG/MCP primary antibodies

  • Description

    Rabbit monoclonal [EPR13194] to Actin Regulatory Protein CAPG/MCP

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, U937 and THP1 cell lysates.

  • General notes

     

     

     This product was previously labelled as Actin Regulatory Protein CAPG

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Actin Regulatory Protein CAPG/MCP antibody [EPR13194] (ab181092)
    Western blot - Anti-Actin Regulatory Protein CAPG/MCP antibody [EPR13194] (ab181092)
    All lanes : Anti-Actin Regulatory Protein CAPG/MCP antibody [EPR13194] (ab181092) at 1/1000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : CAPG knockout HeLa cell lysate
    Lane 3 : THP-1 cell lysate
    Lane 4 : U-937 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 38 kDa
    Observed band size: 48 kDa
    why is the actual band size different from the predicted?



    Lanes 1-4: Merged signal (red and green). Green - ab181092 observed at 48 kDa. Red - loading control ab8245 observed at 36 kDa. 

     ab181092 Anti-Actin Regulatory Protein CAPG/MCP antibody [EPR13194] was shown to specifically react with Actin Regulatory Protein CAPG/MCP in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265766 (knockout cell lysate ab257378) was used.  Wild-type and Actin Regulatory Protein CAPG/MCP knockout samples were subjected to SDS-PAGE.  ab181092 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2. 5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunoprecipitation - Anti-Actin Regulatory Protein CAPG/MCP antibody [EPR13194] (ab181092)
    Immunoprecipitation - Anti-Actin Regulatory Protein CAPG/MCP antibody [EPR13194] (ab181092)

    Western blot analysis of THP1 cell lysate immunoprecipitated using ab181092 at 1/60 dilution.
    Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.

  • Western blot - Anti-Actin Regulatory Protein CAPG/MCP antibody [EPR13194] (ab181092)
    Western blot - Anti-Actin Regulatory Protein CAPG/MCP antibody [EPR13194] (ab181092)
    Anti-Actin Regulatory Protein CAPG/MCP antibody [EPR13194] (ab181092) at 1/10000 dilution + THP1 cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 38 kDa
    Observed band size: 39 kDa why is the actual band size different from the predicted?

  • Western blot - Anti-Actin Regulatory Protein CAPG/MCP antibody [EPR13194] (ab181092)
    Western blot - Anti-Actin Regulatory Protein CAPG/MCP antibody [EPR13194] (ab181092)
    Anti-Actin Regulatory Protein CAPG/MCP antibody [EPR13194] (ab181092) at 1/50000 dilution + U937 cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 38 kDa
    Observed band size: 39 kDa why is the actual band size different from the predicted?

  • Anti-Actin Regulatory Protein CAPG/MCP antibody [EPR13194] (ab181092)
    Anti-Actin Regulatory Protein CAPG/MCP antibody [EPR13194] (ab181092)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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