Antibodies, Proteins, Kits and Reagents for Life Science

291 484 ₸ Product size

100 µl 291 484 ₸

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Anti-Actin Regulatory Protein CAPG/MCP antibody [EPR13194] (ab181092)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR13194] to Actin Regulatory Protein CAPG/MCP
Suitable for: WB, IP
Knockout validated
Reacts with: Human

Product name

Anti-Actin Regulatory Protein CAPG/MCP antibody [EPR13194]
See all Actin Regulatory Protein CAPG/MCP primary antibodies
Description

Rabbit monoclonal [EPR13194] to Actin Regulatory Protein CAPG/MCP
Host species

Rabbit
Tested Applications & Species

Application Species
IP
Human

WB
Human

See all applications and species data
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HeLa, U937 and THP1 cell lysates.
General notes
This product was previously labelled as Actin Regulatory Protein CAPG

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IP	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR13194
Isotype

IgG
Research areas

Western blot - Anti-Actin Regulatory Protein CAPG/MCP antibody [EPR13194] (ab181092)

All lanes : Anti-Actin Regulatory Protein CAPG/MCP antibody [EPR13194] (ab181092) at 1/1000 dilution

Lane 1 : Wild-type HeLa cell lysate
Lane 2 : CAPG knockout HeLa cell lysate
Lane 3 : THP-1 cell lysate
Lane 4 : U-937 cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

Predicted band size: 38 kDa
Observed band size: 48 kDa
why is the actual band size different from the predicted?

Lanes 1-4: Merged signal (red and green). Green - ab181092 observed at 48 kDa. Red - loading control ab8245 observed at 36 kDa.

ab181092 Anti-Actin Regulatory Protein CAPG/MCP antibody [EPR13194] was shown to specifically react with Actin Regulatory Protein CAPG/MCP in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265766 (knockout cell lysate ab257378) was used. Wild-type and Actin Regulatory Protein CAPG/MCP knockout samples were subjected to SDS-PAGE. ab181092 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2. 5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Immunoprecipitation - Anti-Actin Regulatory Protein CAPG/MCP antibody [EPR13194] (ab181092)

Western blot analysis of THP1 cell lysate immunoprecipitated using ab181092 at 1/60 dilution.
Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.
Western blot - Anti-Actin Regulatory Protein CAPG/MCP antibody [EPR13194] (ab181092)

Anti-Actin Regulatory Protein CAPG/MCP antibody [EPR13194] (ab181092) at 1/10000 dilution + THP1 cell lysate at 10 µg

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 38 kDa
Observed band size: 39 kDa why is the actual band size different from the predicted?
Western blot - Anti-Actin Regulatory Protein CAPG/MCP antibody [EPR13194] (ab181092)

Anti-Actin Regulatory Protein CAPG/MCP antibody [EPR13194] (ab181092) at 1/50000 dilution + U937 cell lysate at 20 µg

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 38 kDa
Observed band size: 39 kDa why is the actual band size different from the predicted?
Anti-Actin Regulatory Protein CAPG/MCP antibody [EPR13194] (ab181092)

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