Anti-Actin Regulatory Protein CAPG/MCP antibody (ab90873)
Key features and details
- Rabbit polyclonal to Actin Regulatory Protein CAPG/MCP
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Actin Regulatory Protein CAPG/MCP antibody
See all Actin Regulatory Protein CAPG/MCP primary antibodies -
Description
Rabbit polyclonal to Actin Regulatory Protein CAPG/MCP -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide corresponding to Human Actin Regulatory Protein CAPG/MCP aa 100-200 conjugated to keyhole limpet haemocyanin.
(Peptide available asab101225) -
Positive control
- Recombinant Human Actin Regulatory Protein CAPG/MCP (ab95385) can be used as a positive control in WB. This antibody gave a positive signal in the following whole cell lysates: HL60; U937; THP1; RAW 264.7.
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General notes
This product was previously labelled as Actin Regulatory Protein CAPG
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab90873 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB HumanAll applications RatCowApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa).Target
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Function
Calcium-sensitive protein which reversibly blocks the barbed ends of actin filaments but does not sever preformed actin filaments. May play an important role in macrophage function. May play a role in regulating cytoplasmic and/or nuclear structures through potential interactions with actin. May bind DNA. -
Tissue specificity
Macrophages and macrophage-like cells. -
Sequence similarities
Belongs to the villin/gelsolin family.
Contains 3 gelsolin-like repeats. -
Post-translational
modificationsThe N-terminus is blocked. -
Cellular localization
Cytoplasm. Nucleus. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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Database links
- Entrez Gene: 353121 Cow
- Entrez Gene: 822 Human
- Entrez Gene: 12332 Mouse
- Entrez Gene: 297339 Rat
- Omim: 153615 Human
- SwissProt: Q865V6 Cow
- SwissProt: P40121 Human
- SwissProt: P24452 Mouse
see all -
Alternative names
- Actin capping protein GCAP39 antibody
- Actin regulatory protein CAP G antibody
- Actin regulatory protein CAP-G antibody
see all
Images
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Western blot - Anti-Actin Regulatory Protein CAPG/MCP antibody (ab90873)All lanes : Anti-Actin Regulatory Protein CAPG/MCP antibody (ab90873) at 1 µg/ml
Lane 1 : HL60 (Human promyelocytic leukemia cell line) Whole Cell Lysate
Lane 2 : U937 (Human leukemic monocyte lymphoma cell line) Whole Cell Lysate
Lane 3 : THP1 (Human acute monocytic leukemia cell line) Whole Cell Lysate
Lane 4 : RAW 264.7 (Mouse leukaemic monocyte macrophage cell line) Whole Cell Lysate
Lysates/proteins at 10 µg/ml per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 39 kDa
Observed band size: 39 kDa
Additional bands at: 36 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutes
Datasheets and documents
References (0)
ab90873 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-Actin Regulatory Protein CAPG/MCP antibody (ab90873)All lanes : Anti-Actin Regulatory Protein CAPG/MCP antibody (ab90873) at 1 µg/ml
Lane 1 : HL60 (Human promyelocytic leukemia cell line) Whole Cell Lysate
Lane 2 : U937 (Human leukemic monocyte lymphoma cell line) Whole Cell Lysate
Lane 3 : THP1 (Human acute monocytic leukemia cell line) Whole Cell Lysate
Lane 4 : RAW 264.7 (Mouse leukaemic monocyte macrophage cell line) Whole Cell Lysate
Lysates/proteins at 10 µg/ml per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 39 kDa
Observed band size: 39 kDa
Additional bands at: 36 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutes
