Recombinant human Caspase-8 protein (ab54117)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Active: Yes
- Tags: His tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies, WB
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Product name
Recombinant human Caspase-8 protein
See all Caspase-8 proteins and peptides -
Biological activity
Minimum Specific Activity: 5000 units/mg. One unit of the recombinant Caspase-8 is the enzyme activity that cleaves 1 nmol of the caspase substrate IETD-pNA (pNA: pnitroanaline) per hour at 37°C in a reaction solution containing 50 mM Hepes, pH 7.2, 50 mM NaCl, 0.1% Chaps, 10 mM EDTA, 5% Glycerol, and 10 mM DTT.
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Purity
> 95 % SDS-PAGE. -
Expression system
Escherichia coli -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Tags
His tag N-Terminus
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Preparation and Storage
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Stability and Storage
Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
Constituents: PBS, 15% Glycerol
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionReconstitute to 1 unit per µl in PBS containing 15% glycerol.
Images
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Western blot - Recombinant human Caspase-8 protein (ab54117)
Sample: Recombinant caspase 8 protein ab54117 at 100 ng. Primary antibody: Cleaved caspase 8 at 1:1000. Secondary antibody: Goat polyclonal to Rabbit IgG H&L at 1:10000. Development: ECL for exposure 10 min. Predicted band size: 18 kDa. Observed band size: 24 kDa.
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Western blot - Recombinant human Caspase-8 protein (ab54117)
