Astana Biomed Group, an authorized Abcam distributor in Central Asia

Recombinant human BTC protein (ab200256)

Key features and details

  • Expression system: Escherichia coli
  • Purity: > 98% SDS-PAGE
  • Endotoxin level:
  • Active: Yes
  • Suitable for: SDS-PAGE, HPLC, Functional Studies

Description

  • Product name

    Recombinant human BTC protein
    See all BTC proteins and peptides

  • Biological activity

    The ED50 was determined by the dose-dependent stimulation of the proliferation of murine Balb/3T3 cells is ≤ 0.05 ng/ml, corresponding to a specific activity of ≥ 2 x 107units/mg.

  • Purity

    > 98 % SDS-PAGE.
    The purity of ab170516 was >98% by SDS-PAGE and HPLC analyses.

  • Endotoxin level

  • Expression system

    Escherichia coli

  • Accession

    P35070

  • Protein length

    Full length protein

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Sequence

      DGNSTRSPET NGLLCGDPEE NCAATTTQSK RKGHFSRCPK QYKHYCIKGR CRFVVAEQTP SCVCDEGYIG ARCERVDLFY

    • Predicted molecular weight

      8 kDa

    • Amino acids

      32 to 111

    • Additional sequence information

      This product is for the mature, full length Betacellulin protein from aa 32 to 111. The signal peptide is not included

    • Associated products

      Specifications

      Our Abpromise guarantee covers the use of ab200256 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      • Applications

        SDS-PAGE

        HPLC

        Functional Studies

      • Form

        Lyophilized

      • Additional notes

        Protein previously labeled as Betacellulin.

      • Concentration information loading...

      Preparation and Storage

      • Stability and Storage

        Shipped at 4°C. Store at -20°C long term. Avoid freeze / thaw cycle.

        pH: 7.40
        Constituent: 100% PBS

        Lyophilized from a 0.2 um filtered concentrated solution.

        This product is an active protein and may elicit a biological response in vivo, handle with caution.

      • Reconstitution
        Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL

      General Info

      • Alternative names

        • Betacellulin
        • Betacellulin precursor
        • BTC
        • BTC_HUMAN
        • OTTHUMP00000160600
        • OTTHUMP00000219057
        • Probetacellulin
        see all

      • Function

        Potent mitogen for retinal pigment epithelial cells and vascular smooth muscle cells. The effects of betacellulin are probably mediated by the EGF receptor and other related receptors.

      • Tissue specificity

        Synthesized in several tissues and tumor cells. Predominantly expressed in pancreas and small intestine.

      • Sequence similarities

        Contains 1 EGF-like domain.

      • Cellular localization

        Cell membrane and Secreted > extracellular space.
      • Information by UniProt

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab200256? Please let us know so that we can cite the reference in this datasheet.

    ab200256 has not yet been referenced specifically in any publications.

    Preparation and Storage

    • Alternative names

      • Betacellulin
      • Betacellulin precursor
      • BTC
      • BTC_HUMAN
      • OTTHUMP00000160600
      • OTTHUMP00000219057
      • Probetacellulin
      see all

    • Function

      Potent mitogen for retinal pigment epithelial cells and vascular smooth muscle cells. The effects of betacellulin are probably mediated by the EGF receptor and other related receptors.

    • Tissue specificity

      Synthesized in several tissues and tumor cells. Predominantly expressed in pancreas and small intestine.

    • Sequence similarities

      Contains 1 EGF-like domain.

    • Cellular localization

      Cell membrane and Secreted > extracellular space.
    • Information by UniProt

    Images

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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