Recombinant human Acetyl Coenzyme A Carboxylase protein (ab196428)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: >= 70% SDS-PAGE
- Active: Yes
- Tags: His-DDDDK tag C-Terminus
- Suitable for: SDS-PAGE, Functional Studies
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Product name
Recombinant human Acetyl Coenzyme A Carboxylase protein
See all Acetyl Coenzyme A Carboxylase proteins and peptides -
Biological activity
Specific Activity: ≥42.5 pmol/min/µg.
A 25 μl Acetyl Coenzyme A carboxylase alpha reaction is conducted in a buffer containing 30 mM HEPES (pH 7.4), 2 mM MgCl2, 2 mM potassium citrate, 1 mM DTT, 100 μM ATP, 12 mM NaHCO3 and 20 μM acetyl-CoA at 30°C for 45 min. ATP reduction is detected.
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Purity
>= 70 % SDS-PAGE. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MWWSTLMSILRARSFWKWISTQTVRIIRAVRAHFGGIMDEPSPLAQPLEL NQHSRFIIGSVSEDNSEDEISNLVKLDLLEEKEGSLSPASVGSDTLSDLG ISSLQDGLALHIRSSMSGLHLVKQGRDRKKIDSQRDFTVASPAEFVTRFG GNKVIEKVLIANNGIAAVKCMRSIRRWSYEMFRNERAIRFVVMVTPEDLK ANAEYIKMADHYVPVPGGPNNNNYANVELILDIAKRIPVQAVWAGWGHAS ENPKLPELLLKNGIAFMGPPSQAMWALGDKIASSIVAQTAGIPTLPWSGS GLRVDWQENDFSKRILNVPQELYEKGYVKDVDDGLQAAEEVGYPVMIKAS EGGGGKGIRKVNNADDFPNLFRQVQAEVPGSPIFVMRLAKQSRHLEVQIL ADQYGNAISLFGRDCSVQRRHQKIIEEAPATIATPAVFEHMEQCAVKLAK MVGYVSAGTVEYLYSQDGSFYFLELNPRLQVEHPCTEMVADVNLPAAQLQ IAMGIPLYRIKDIRMMYGVSPWGDSPIDFEDSAHVPCPRGHVIAARITSE NPDEGFKPSSGTVQELNFRSNKNVWGYFSVAAAGGLHEFADSQFGHCFSW GENREEAISNMVVALKELSIRGDFRTTVEYLIKLLETESFQMNRIDTGWL DRLIAEKVQAERPDTMLGVVCGALHVADVSLRNSVSNFLHSLERGQVLPA HTLLNTVDVELIYEGVKYVLKVTRQSPNSYVVIMNGSCVEVDVHRLSDGG LLLSYDGSSYTTYMKEEVDRYRITIGNKTCVFEKENDPSVMRSPSAGKLI QYIVEDGGHVFAGQCYAEIEVMKMVMTLTAVESGCIHYVKRPGAALDPGC VLAKMQLDNPSKVQQAELHTGSLPRIQSTALRGEKLHRVFHYVLDNLVNV MNGYCLPDPFFSSKVKDWVERLMKTLRDPSLPLLELQDIMTSVSGRIPPN VEKSIKKEMAQYASNITSVLCQFPSQQIANILDSHAATLNRKSEREVFFM NTQSIVQLVQRYRSGIRGHMKAVVMDLLRQYLRVETQFQNGHYDKCVFAL REENKSDMNTVLNYIFSHAQVTKKNLLVTMLIDQLCGRDPTLTDELLNIL TELTQLSKTTNAKVALRARQVLIASHLPSYELRHNQVESIFLSAIDMYGH QFCIENLQKLILSETSIFDVLPNFFYHSNQVVRMAALEVYVRRAYIAYEL NSVQHRQLKDNTCVVEFQFMLPTSHPNRGNIPTLNRMSFSSNLNHYGMTH VASVSDVLLDNSFTPPCQRMGGMVSFRTFEDFVRIFDEVMGCFSDSPPQS PTFPEAGHTSLYDEDKVPRDEPIHILNVAIKTDCDIEDDRLAAMFREFTQ QNKATLVDHGIRRLTFLVAQKDFRKQVNYEVDRRFHREFPKFFTFRARDK FEEDRIYRHLEPALAFQLELNRMRNFDLTAIPCANHKMHLYLGAAKVEVG TEVTDYRFFVRAIIRHSDLVTKEASFEYLQNEGERLLLEAMDELEVAFNN TNVRTDCNHIFLNFVPTVIMDPSKIEESVRSMVMRYGSRLWKLRVLQAEL KINIRLTPTGKAIPIRLFLTNESGYYLDISLYKEVTDSRTAQIMFQAYGD KQGPLHGMLINTPYVTKDLLQSKRFQAQSLGTTYIYDIPEMFRQSLIKLW ESMSTQAFLPSPPLPSDMLTYTELVLDDQGQLVHMNRLPGGNEIGMVAWK MTFKSPEYPEGRDIIVIGNDITYRIGSFGPQEDLLFLRASELARAEGIPR IYVSANSGARIGLAEEIRHMFHVAWVDPEDPYKGYRYLYLTPQDYKRVSA LNSVHCEHVEDEGESRYKITDIIGKEEGIGPENLRGSGMIAGESSLAYNE IITISLVTCRAIGIGAYLVRLGQRTIQVENSHLILTGAGALNKVLGREVY TSNNQLGGIQIMHNNGVTHCTVCDDFEGVFTVLHWLSYMPKSVHSSVPLL NSKDPIDRIIEFVPTKTPYDPRWMLAGRPHPTQKGQWLSGFFDYGSFSEI MQPWAQTVVVGRARLGGIPVGVVAVETRTVELSIPADPANLDSEAKIIQQ AGQVWFPDSAFKTYQAIKDFNREGLPLMVFANWRGFSGGMKDMYDQVLKF GAYIVDGLRECCQPVLVYIPPQAELRGGSWVVIDSSINPRHMEMYADRES RGSVLEPEGTVEIKFRRKDLVKTMRRVDPVYIHLAERLGTPELSTAERKE LENKLKEREEFLIPIYHQVAVQFADLHDTPGRMQEKGVISDILDWKTSRT FFYWRLRRLLLEDLVKKKIHNANPELTDGQIQAMLRRWFVEVEGTVKAYV WDNNKDLAEWLEKQLTEEDGVHSVIEENIKCISRDYVLKQIRSLVQANPE VAMDSIIHMTQHISPTQRAEVIRILSTMDSPSTDYKDDDDKHHHHHH -
Predicted molecular weight
270 kDa including tags -
Amino acids
1 to 2383 -
Tags
His-DDDDK tag C-Terminus -
Additional sequence information
NM_198834.
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Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.79% Tris HCl, 2.9% Sodium chloride, 10% Glycerol (glycerin, glycerine), 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Contains 100 µg/ml DDDDK peptide.This product is an active protein and may elicit a biological response in vivo, handle with caution.
Images
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SDS-PAGE - Recombinant human Acetyl Coenzyme A Carboxylase protein (ab196428)
4-20% SDS-PAGE analysis of 2 µg ab196428 with Coomassie staining.
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Functional Studies - Recombinant human Acetyl Coenzyme A Carboxylase protein (ab196428)Activity assay of ab196428.
