Recombinant human 4-1BBL protein (Tagged) (ab216189)
Key features and details
- Expression system: HEK 293 cells
- Purity: >= 90% SDS-PAGE
- Active: Yes
- Tags: His tag N-Terminus
- Suitable for: SDS-PAGE
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Product name
Recombinant human 4-1BBL protein (Tagged)
See all 4-1BBL proteins and peptides -
Biological activity
Assay conditions: Reaction done according to CD137[Biotinylated]:CD137L Inhibitor Screening Assay protocol. First, CD137L is coated 100 ng/well at 4°C. Next, CD137-biotin (0-13.5 ng/ul, 0.5 ng/ul) is incubated with CD137L on the plate for 2 hours at room temperature. Finally, the plate is treated with streptavidin-HRP for 1 hour at room temperature, followed by addition of an HRP substrate. Chemiluminescence produced was be measured using a chemiluminescence reader. Buffer is PBS + 0.1% BSA. Blocking buffer is Superblock + 0.05% Tween-20.
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Purity
>= 90 % SDS-PAGE. -
Expression system
HEK 293 cells -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
ACPWAVSGAR ASPGSAASPR LREGPELSPD DPAGLLDLRQ GMFAQLVAQN VLLIDGPLSW YSDPGLAGVS LTGGLSYKED TKELVVAKAG VYYVFFQLEL RRVVAGEGSG SVSLALHLQP LRSAAGAAAL ALTVDLPPAS SEARNSAFGF QGRLLHLSAG QRLGVHLHTE ARARHAWQLT QGATVLGLFR VTPEIPAGLP SPRSE -
Predicted molecular weight
24 kDa including tags -
Amino acids
50 to 254 -
Tags
His tag N-Terminus -
Additional sequence information
Genbank: NM_003811
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Preparation and Storage
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Alternative names
- 4 1BB L
- 4 1BB ligand
- 4 1BBL
see all -
Function
Cytokine that binds to TNFRSF9. Induces the proliferation of activated peripheral blood T-cells. May have a role in activation-induced cell death (AICD). May play a role in cognate interactions between T-cells and B-cells/macrophages. -
Tissue specificity
Expressed in brain, placenta, lung, skeletal muscle and kidney. -
Sequence similarities
Belongs to the tumor necrosis factor family. -
Cellular localization
Membrane. - Information by UniProt
Images
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SDS-PAGE - Recombinant Human 4-1BBL protein (Tagged) (ab216189)
SDS-PAGE analysis of ab216189.
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Functional Studies - Recombinant human 4-1BBL protein (Tagged) (ab216189)CD173 biotinylated and ab216189 interaction.