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Epigenetics and Nuclear Signaling Transcription Domain Families Zinc Finger

Recombinant Human DREF protein (ab152028)

Key features and details

  • Expression system: Escherichia coli
  • Purity: > 95% SDS-PAGE
  • Endotoxin level:
  • Tags: His tag C-Terminus
  • Suitable for: SDS-PAGE

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Description

  • Product name

    Recombinant Human DREF protein
  • Purity

    > 95 % SDS-PAGE.

  • Endotoxin level

  • Expression system

    Escherichia coli
  • Accession

    O96006
  • Protein length

    Protein fragment
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Sequence

      NKSLESSQTDLKLVAHPRAKSKVWKYFGFDTNAEGCILQWKKIYCRICMA QIAYSGNTSNLSYHLEKNHPEEFCEFVKSNTEQMREAFATAFSKLKPE
    • Predicted molecular weight

      11 kDa
    • Amino acids

      3 to 100
    • Tags

      His tag C-Terminus
  • Associated products

    • Related Products

      • Anti-6X His tag® antibody [HIS.H8] (ab18184)
      • Anti-6X His tag® antibody [4D11] (ab5000)
      • Anti-6X His tag® antibody (ab9108)

    Specifications

    Our Abpromise guarantee covers the use of ab152028 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      SDS-PAGE

    • Form

      Lyophilized
    • Additional notes

      Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at

    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped on Dry Ice. The lyophilized protein is stable for a few weeks at room temperature. Store at -20°C. Please see notes section.

      pH: 7.20
      Constituents: 99% Phosphate Buffer, 0.88% Sodium chloride

    • Reconstitution
      Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 µg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

    General Info

    • Alternative names

      • Ac like transposable element
      • ALTE
      • BED type zinc finger domain containing protein 1
      • DNA replication related element binding factor
      • dREF homolog
      • hDREF
      • KIAA0785
      • Putative Ac like transposon
      • Putative Ac-like transposable element
      • TRAMP
      • ZBED1
      • ZBED1_HUMAN
      • Zinc finger BED domain-containing protein 1
      • Zinc finger BED type containing 1
      see all
    • Function

      Binds to 5'-TGTCG[CT]GA[CT]A-3' DNA elements found in the promoter regions of a number of genes related to cell proliferation. Binds to the histone H1 promoter and stimulates transcription. Was first identified as gene weakly similar to Ac transposable elements, but does not code for any transposase activity.
    • Tissue specificity

      Ubiquitously expressed at low levels. Expression is highest in skeletal muscle, heart, spleen and placenta.
    • Sequence similarities

      Contains 1 BED-type zinc finger.
    • Cellular localization

      Nucleus. In granular structures.
    • Target information above from: UniProt accession O96006 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab152028? Please let us know so that we can cite the reference in this datasheet.

    ab152028 has not yet been referenced specifically in any publications.

    Preparation and Storage

    • Stability and Storage

      Shipped on Dry Ice. The lyophilized protein is stable for a few weeks at room temperature. Store at -20°C. Please see notes section.

      pH: 7.20
      Constituents: 99% Phosphate Buffer, 0.88% Sodium chloride

    • Reconstitution
      Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 µg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

    Images

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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