Rat uPA ELISA kit (total uPA antigen) (ab272781)
Key features and details
- Range: 0.01 ng/ml - 10 ng/ml
- Sample type: Cell culture media, Cit plasma, EDTA Plasma, Serum, Tissue Extracts, Urine
- Detection method: Colorimetric
- Assay type: Quantitative
- Reacts with: Rat
Overview
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Product name
Rat uPA ELISA kit (total uPA antigen)
See all uPA kits -
Detection method
Colorimetric -
Sample type
Urine, Serum, Tissue Extracts, Cell culture media, EDTA Plasma, Cit plasma -
Assay type
Quantitative -
Range
0.01 ng/ml - 10 ng/ml -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Rat -
Product overview
Rat uPA ELISA kit (total uPA antigen) (ab272781) is intended for the quantitative determination of total urokinase plasminogen activator in rat plasma, serum, urine, cell culture media, or tissue extracts.
Rat uPA will bind to the capture antibody coated on the microtiter plate. Free and complexed enzyme will react with the capture antibody on the plate. After appropriate washing steps, polyclonal anti-rat uPA primary antibody binds to the captured enzyme. Excess antibody is washed away and bound polyclonal antibody is then reacted with the secondary antibody conjugated to horseradish peroxidase. TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of uPA.
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Tested applications
Suitable for: ELISAmore details -
Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Wash Buffer 1 x 50ml Anti-Rabbit Streptavidin-HRP Secondary Reagent 1 vial Anti-Rat uPA Primary Antibody Lyophilized Vial 1 vial Rat uPA ELISA Plate 1 unit Rat uPA Standard Lyophilized Vail 1 vial TMB Substrate 1 x 10ml -
Research areas
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Relevance
Urokinase type plasminogen activator (uPA) is secreted from cells as a precursor which is activated to the two chain form consisting of an A and B chain. The active high MW form is further processed by removal of an amino terminal fragment to an active low MW form (35 kDa). uPA is a serine protease that activates plasminogen toplasmin. High levels of uPA and plasminogen activator inhibitor type 1 (PAI 1) in breast cancer tissue extracts have been associated with rapid disease progression. The malignant phenotype of prostatic tumor cells correlates with the expression of both uPA and its cell membrane receptor (uPAR). -
Alternative names
- ATF
- BDPLT5
- EC 3.4.21.73
see all -
Database links
- Entrez Gene: 25619 Rat
- SwissProt: P29598 Rat
- Unigene: 6064 Rat
Images
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Example data
A typical standard curve. Example only.