Rat L-FABP ELISA Kit (ab190545)
Key features and details
- Sensitivity: 1.278 ng/ml
- Range: 3.125 ng/ml - 200 ng/ml
- Sample type: Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Rat
Overview
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Product name
Rat L-FABP ELISA Kit
See all liver FABP kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
1.278 ng/ml -
Range
3.125 ng/ml - 200 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum > 85 % - % -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Rat -
Product overview
Abcam’s L-FABP Rat ELISA Kit (ab190545) is an in vitro enzyme-linked immunosorbent assay (ELISA) for the quantitative measurement of L-FABP in rat serum and plasma.
In this assay the L-FABP present in samples reacts with the anti-L-FABP antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-L-FABP antibodies conjugated with horseradish peroxidase (HRP) are added. These enzyme-labeled antibodies form complexes with the previously bound L-FABP. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of L-FABP in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of L-FABP in the test sample. The quantity of L-FABP in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
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Platform
Microplate
Properties
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Components 1 x 96 tests 1X Diluent Concentrate 1 x 60ml 20X Wash Buffer Concentrate 1 x 50ml Chromogen Substrate Solution 1 x 12ml L-FABP Rat Antibody coated microwells 1 x 96 tests L-FABP Rat Calibrator (Lyophilized) 1 vial L-FABP Rat HRP Conjugate 1 vial Stop Solution 1 x 12ml -
Research areas
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Function
Binds free fatty acids and their coenzyme A derivatives, bilirubin, and some other small molecules in the cytoplasm. May be involved in intracellular lipid transport. -
Sequence similarities
Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family. -
Domain
Forms a beta-barrel structure that accommodates hydrophobic ligands in its interior. -
Cellular localization
Cytoplasm. - Information by UniProt
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Alternative names
- FABP 1
- FABP1
- FABPL
see all -
Database links
- Entrez Gene: 24360 Rat
- SwissProt: P02692 Rat
- Unigene: 36412 Rat
Images
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Standard Curve
Representative standard curve using ab190545 L-FABP Rat ELISA Kit.
