Rat Cardiac Troponin I ELISA Kit (ab246529)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 7.7 pg/ml
- Range: 15.63 pg/ml - 1250 pg/ml
- Sample type: EDTA Plasma, Serum, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Rat
Overview
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Product name
Rat Cardiac Troponin I ELISA Kit
See all Cardiac Troponin I kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 5 3.1% Inter-assay Sample n Mean SD CV% Serum 3 5.5% -
Sample type
Serum, Tissue Extracts, EDTA Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
7.7 pg/ml -
Range
15.63 pg/ml - 1250 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 101 89% - 121% Tissue Extracts 110 99% - 121% EDTA Plasma 94 89% - 99% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Rat -
Product overview
Rat Cardiac Troponin I ELISA Kit (ab246529) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Cardiac Troponin I protein in serum, tissue extracts, and edta plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Rat Cardiac Troponin I with 7.7 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Rat Cardiac Troponin I Capture Antibody 1 x 600µl 10X Rat Cardiac Troponin I Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent CPR 1 x 6ml Plate Seals 1 unit Rat Cardiac Troponin I Lyophilized Recombinant Protein 2 vials Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. -
Involvement in disease
Defects in TNNI3 are the cause of cardiomyopathy familial hypertrophic type 7 (CMH7) [MIM:613690]. Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.
Defects in TNNI3 are the cause of cardiomyopathy familial restrictive type 1 (RCM1) [MIM:115210]. RCM1 is an heart muscle disorder characterized by impaired filling of the ventricles with reduced diastolic volume, in the presence of normal or near normal wall thickness and systolic function.
Defects in TNNI3 are the cause of cardiomyopathy dilated type 2A (CMD2A) [MIM:611880]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
Defects in TNNI3 are the cause of cardiomyopathy dilated type 1FF (CMD1FF) [MIM:613286]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death. -
Sequence similarities
Belongs to the troponin I family. - Information by UniProt
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Alternative names
- cardiac muscle
- Cardiac troponin I
- cardiomyopathy, dilated 2A (autosomal recessive)
see all -
Database links
- Entrez Gene: 29248 Rat
- SwissProt: P23693 Rat
- Unigene: 64141 Rat
Images
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Other - Rat Cardiac Troponin 1 ELISA Kit (ab246529)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of rat Cardiac Troponin I standard curve in Sample Diluent NS.The Cardiac Troponin I standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Example of rat Cardiac Troponin I standard curve in 1X Cell Extraction Buffer PTR.The Cardiac Troponin I standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native Cardiac Troponin I in rat heart homogenate extract based on a 333.3 pg/mL extract load.The concentrations of Cardiac Troponin I were measured in duplicate and interpolated from the Cardiac Troponin I standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Cardiac Troponin I concentration was determined to be 857.0 pg/mL in heart homogenate extract.
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Sandwich ELISA - Rat Cardiac Troponin I ELISA Kit (ab246529)To learn more about the advantages of recombinant antibodies see here.
