Sandwich ELISA using ab238617 as the capture antibody at 200ng/well, and a Biotinylated anti-human light chains (κ+ λ) antibody as the detection antibody, followed by an AP conjugated streptavidin.

Sandwich ELISA using ab238617 as the capture antibody, and a biotinylated anti-human light chains ( κ+ λ) antibody as the detection antibody, followed by an AP conjugated streptavidin.

Paraffin-embedded human lymphoid tissue stained for IgG4 heavy chain using ab238617 at 10 μg/mL in immunohistochemical analysis.

A titer ELISA using ab238617. The plate was coated with different amounts of human IgG4. A serial dilution of ab238617 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG was used as the secondary antibody.

All lanes : Rabbit monoclonal [RM120] Anti-Human IgG4 heavy chain (Biotin) (ab238617) at 2 µg/ml

Lane 1 : Non-reduced (-) and reduced (+) forms of Human IgG1
Lane 2 : Non-reduced (-) and reduced (+) forms of Human IgG2
Lane 3 : Non-reduced (-) and reduced (+) forms of Human IgG3
Lane 4 : Non-reduced (-) and reduced (+) forms of Human IgG4
Lane 5 : Non-reduced (-) and reduced (+) forms of Mouse IgG
Lane 6 : Non-reduced (-) and reduced (+) forms of Rat IgG
Lane 7 : Non-reduced (-) and reduced (+) forms of Goat IgG

Predicted band size: 36 kDa

Western Blot of human, mouse, rat, and goat IgG shows RM120 reacts to human IgG4, in both whole molecule (~150kDa, non-reduced) and heavy chain (~50kDa, reduced) forms (Lane 4). No cross reactivity with other isotypes of human IgG, or mouse, rat, or goat IgG.