Antibodies, Proteins, Kits and Reagents for Life Science

Rabbit monoclonal [RM120] Anti-Human IgG4 heavy chain (Biotin) (ab238617)

Key features and details

Rabbit monoclonal [RM120] Anti-Human IgG4 heavy chain (Biotin)
Conjugation: Biotin
Host species: Rabbit
Isotype: IgG
Suitable for: IHC-P, WB, Sandwich ELISA

Product name

Rabbit monoclonal [RM120] Anti-Human IgG4 heavy chain (Biotin)
See all IgG4 secondary antibodies
Host species

Rabbit
Target species

Human
Specificity

ab238617 reacts to the heavy chain of human IgG4. No cross reactivity with human IgG1, IgG2, IgG3, IgM, IgA, IgD, IgE, mouse IgG, rat IgG, or goat IgG.
Tested applications

Suitable for: IHC-P, WB, Sandwich ELISAmore details
Immunogen

Synthetic peptide corresponding to Human IgG4 (Hinge).
Database link: P01861
Conjugation

Biotin

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark.
Storage buffer

Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol, 1% BSA
Concentration information loading...
Purity

Protein A purified
Purification notes

Purified from an animal origin–free culture supernatant.
Clonality

Monoclonal
Clone number

RM120
Isotype

IgG
Research areas

Sandwich ELISA - Rabbit monoclonal [RM120] Anti-Human IgG4 heavy chain (Biotin) (ab238617)

Sandwich ELISA using ab238617 as the capture antibody at 200ng/well, and a Biotinylated anti-human light chains (κ+ λ) antibody as the detection antibody, followed by an AP conjugated streptavidin.
Sandwich ELISA - Rabbit monoclonal [RM120] Anti-Human IgG4 heavy chain (Biotin) (ab238617)

Sandwich ELISA using ab238617 as the capture antibody, and a biotinylated anti-human light chains ( κ+ λ) antibody as the detection antibody, followed by an AP conjugated streptavidin.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit monoclonal [RM120] Anti-Human IgG4 heavy chain (Biotin) (ab238617)

Paraffin-embedded human lymphoid tissue stained for IgG4 heavy chain using ab238617 at 10 μg/mL in immunohistochemical analysis.
ELISA - Rabbit monoclonal [RM120] Anti-Human IgG4 heavy chain (Biotin) (ab238617)

A titer ELISA using ab238617. The plate was coated with different amounts of human IgG4. A serial dilution of ab238617 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG was used as the secondary antibody.
Western blot - Rabbit monoclonal [RM120] Anti-Human IgG4 heavy chain (Biotin) (ab238617)

All lanes : Rabbit monoclonal [RM120] Anti-Human IgG4 heavy chain (Biotin) (ab238617) at 2 µg/ml

Lane 1 : Non-reduced (-) and reduced (+) forms of Human IgG1
Lane 2 : Non-reduced (-) and reduced (+) forms of Human IgG2
Lane 3 : Non-reduced (-) and reduced (+) forms of Human IgG3
Lane 4 : Non-reduced (-) and reduced (+) forms of Human IgG4
Lane 5 : Non-reduced (-) and reduced (+) forms of Mouse IgG
Lane 6 : Non-reduced (-) and reduced (+) forms of Rat IgG
Lane 7 : Non-reduced (-) and reduced (+) forms of Goat IgG

Predicted band size: 36 kDa

Western Blot of human, mouse, rat, and goat IgG shows RM120 reacts to human IgG4, in both whole molecule (~150kDa, non-reduced) and heavy chain (~50kDa, reduced) forms (Lane 4). No cross reactivity with other isotypes of human IgG, or mouse, rat, or goat IgG.

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