Rabbit monoclonal [RM108] Anti-Mouse IgG2b Fc (Biotin) (ab238532)
![Rabbit monoclonal [RM108] Anti-Mouse IgG2b Fc (Biotin) (ab238532)](https://www.abcam.com/ps/products/238/ab238532/Images/ab238532-316416-ab238532WB.jpg "Rabbit monoclonal [RM108] Anti-Mouse IgG2b Fc (Biotin) (ab238532)")
Key features and details
- Rabbit monoclonal [RM108] Anti-Mouse IgG2b Fc (Biotin)
- Conjugation: Biotin
- Host species: Rabbit
- Isotype: IgG
- Suitable for: WB, ELISA
Overview
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Product name
Rabbit monoclonal [RM108] Anti-Mouse IgG2b Fc (Biotin) -
Host species
Rabbit -
Target species
Mouse -
Specificity
ab238532 reacts to the Fc region of mouse IgG2b. No cross reactivity with mouse IgG1, IgG2a, IgG3, IgM, IgA, IgE, human IgG, rat IgG, or goat IgG.
The Fc region of RM108 has been engineered to eliminate Fc receptor binding. -
Tested applications
Suitable for: WB, ELISAmore details -
Immunogen
Full length protein corresponding to Mouse IgG2b.
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Conjugation
Biotin
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
ab238532 was purified from an animal origin–free culture supernatant. -
Clonality
Monoclonal -
Clone number
RM108 -
Isotype
IgG -
Research areas
Images
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Western blot - Rabbit monoclonal [RM108] Anti-Mouse IgG2b Fc (Biotin) (ab238532)All lanes : Rabbit monoclonal [RM108] Anti-Mouse IgG2b Fc (Biotin) (ab238532) at 0.2 µg/ml
Lane 1 : Non-reduced mouse IgG2b
Lane 2 : Reduced mouse IgG2b
Lysates/proteins at 20 µg per lane.
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![ELISA - Rabbit monoclonal [RM108] Anti-Mouse IgG2b Fc (Biotin) (ab238532)](https://www.abcam.com/ps/products/238/ab238532/Images/ab238532-316415-ab2385322.jpg)
ELISA - Rabbit monoclonal [RM108] Anti-Mouse IgG2b Fc (Biotin) (ab238532)ELISA of mouse immunoglobulins shows ab238532 reacts to the Fc region of mouse IgG2b; no cross reactivity with IgG1, IgG2a, IgG3, IgM, IgA, IgE, human IgG, rat IgG, or goat IgG. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL of ab238532 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
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![ELISA - Rabbit monoclonal [RM108] Anti-Mouse IgG2b Fc (Biotin) (ab238532)](https://www.abcam.com/ps/products/238/ab238532/Images/ab238532-316414-ab238532.jpg)
ELISA - Rabbit monoclonal [RM108] Anti-Mouse IgG2b Fc (Biotin) (ab238532)A titer ELISA of mouse IgG2b. The plate was coated with different amounts of mouse IgG2b. A serial dilution of ab238532 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.