Protein Carbonyl ELISA Kit (ab238536)
Key features and details
- Assay type: Cell-based (quantitative)
- Detection method: Colorimetric
- Platform: Microplate reader
- Sample type: Cell Lysate, EDTA Plasma, Hep Plasma, Serum, Tissue Lysate
Overview
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Product name
Protein Carbonyl ELISA Kit
See all Protein Carbonyl kits -
Detection method
Colorimetric -
Sample type
Serum, Cell Lysate, Hep Plasma, EDTA Plasma, Tissue Lysate -
Assay type
Cell-based (quantitative) -
Product overview
Protein Carbonyl ELISA Kit (ab238536) is designed for the quantitative measurement of protein carbonyls in plasma, serum, cell lysates or purified proteins.
With this kit protein samples are first allowed to adsorb to wells of a 96-well plate and then react with dinitrophenylhydrazine (DNPH), which allows for derivatization of the carbonyl group. There are no concentration or precipitation steps, that contribute to sample loss. The kit allows detection of as little as 10 µg/mL using a standard microplate reader. The quantity of protein carbonyls in protein sample is determined by comparing its absorbance with that of a known reduced/oxidized BSA standard curve.
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Notes
Related products
Other Protein Carbonyl Content assays include:
- Protein Carbonyl Content Assay (DNPH) ab126287 (most popular protein carbonyl content assay)
- Protein Carbonyl Content Assay (Fluorometric) ab235631
- Protein Carbonyl Content Western Blot Assay ab178020Review the oxidative stress marker and assay guide to learn about more assays for oxidative stress.
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Platform
Microplate reader
Properties
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Storage instructions
Please refer to protocols. -
Components 96 tests 32 tests 10X Wash Buffer 1 x 100ml 1 x 30ml 25X DNPH Solution 1 x 500µl 1 x 500µl 2X DNPH Diluent 1 x 15ml 1 x 15ml Anti-DNP Antibody (1000X) 1 x 20µl 1 x 5µl Blocking Reagent 1 x 20g 1 x 20g Oxidized BSA Standard 1 x 200µl 1 x 75µl Protein Binding Strip Well Plate 1 unit 1 unit Reduced BSA Standard 1 x 200µl 1 x 75µl Secondary Antibody, HRP Conjugate (1000X) 1 x 20µl 1 x 20µl Stop Solution 1 x 12ml 1 x 4ml Substrate Solution 1 x 12ml 1 x 4ml -
Research areas
Images
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Typical Protein Carbonyl ELISA Standard Curve
This standard curve is for demonstration only. A standard curve must be run with each protein carbonyl elisa experiment.
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Amount of Protein Carbonyl Content for Cell Lysate and BSA Standard.STO (MEF), HeLa and MDA-231 cells were sonicated in 25mM HEPES, pH 7.5, 150 mM NaCl, 10 mM MgCl2, 1 mM EDTA, 2% Glycerol. Cell Lysates and BSA Standard from Pierce BCA Protein Assay were diluted to 10 μg/mL with 1X PBS and coated onto a 96-well Protein Binding Plate. The protein carbonyl levels were determined as described in the Protein Carbonyl ELISA Protocol.