Protein A Detection Elisa Kit (ab273154)
Key features and details
- Range: 64 pg/ml - 4000 pg/ml
- Detection method: Colorimetric
- Assay type: Quantitative
Overview
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Product name
Protein A Detection Elisa Kit -
Detection method
Colorimetric -
Assay type
Quantitative -
Range
64 pg/ml - 4000 pg/ml -
Assay duration
One step assay -
Product overview
The Protein A Detection Elisa Kit (ab273154) provides a quick and simple method to estimate the contamination by protein A from Staphylococcus aureus in a solution of antibodies.
The kit includes ready-to-use reagents necessary to analyze 89 samples in 80 minutes.
Capture antibodies coated on the wells, bind the protein A present in the sample and form complexes that are revealed by an anti-Protein A peroxidase conjugated detector antibody. After washing to remove any non-specific binding, the ready-to-use substrate solution is added to microwells and color develops proportionally to the amount of protein A in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.
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Tested applications
Suitable for: ELISAmore details -
Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10 x 96 tests Detection antibody 1 x 12ml 1 x 120ml Pre-coated microwells strips 1 x 96 tests 10 x 96 tests Protein A Dissociation Buffer 1 x 12ml 1 x 120ml Protein A standard 0.0 pg/ml 1 x 0.3ml 1 x 1ml Protein A standard 1000 pg/ml 1 x 0.3ml 1 x 1ml Protein A standard 125 pg/ml 1 x 0.3ml 1 x 1ml Protein A standard 2000 pg/ml 1 x 0.3ml 1 x 1ml Protein A standard 4000 pg/ml 1 x 0.3ml 1 x 1ml Protein A standard 500 pg/ml 1 x 0.3ml 1 x 1ml Protein A standard 62 pg/ml 1 x 0.3ml 1 x 1ml Sample Diluent 1 x 30ml 1 x 500ml Stop solution 1 x 12ml 1 x 120ml TMB Substrate 1 x 12ml 1 x 120ml
Images
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Protein A Detection Elisa Kit Standard curve
Example standard curve.