Porcine CCL4 ELISA Kit (ab273215)
Key features and details
- Sensitivity: 0.2 ng/ml
- Range: 0.205 ng/ml - 50 ng/ml
- Sample type: Cell culture supernatant, Plasma, Tissue
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Pig
Overview
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Product name
Porcine CCL4 ELISA Kit
See all CCL4/MIP-1 beta kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Plasma, Tissue -
Assay type
Sandwich (quantitative) -
Sensitivity
0.2 ng/ml -
Range
0.205 ng/ml - 50 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 111.3 106% - 118% Plasma 104.8 93% - 121% Cell culture media 122.1 115% - 133% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Pig -
Product overview
Porcine CCL4 ELISA Kit (ab273215) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Porcine CCL4 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Porcine CCL4 coated on a 96-well plate. Standards and samples are pipetted into the wells and Porcine CCL4 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Porcine CCL4 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Porcine CCL4 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer 1 x 25ml 5X Assay Diluent 1 x 15ml 600X HRP-Streptavidin Concentrate 1 x 200µl Anti-Porcine CCL4 coated Microplate (12 x 8 wells) 1 unit Biotinylated Anti-Porcine CCL4 Antibody 2 vials Porcine CCL4 Standard (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB Substrate Solution 1 x 12ml -
Research areas
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Function
Monokine with inflammatory and chemokinetic properties. Binds to CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-beta induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form MIP-1-beta(3-69) retains the abilities to induce down-modulation of surface expression of the chemokine receptor CCR5 and to inhibit the CCR5-mediated entry of HIV-1 in T-cells. MIP-1-beta(3-69) is also a ligand for CCR1 and CCR2 isoform B. -
Sequence similarities
Belongs to the intercrine beta (chemokine CC) family. -
Post-translational
modificationsN-terminal processed form MIP-1-beta(3-69) is produced by proteolytic cleavage after secretion from peripheral blood lymphocytes. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- MIP 1 beta
- Secreted protein G 26
- ACT 2
see all
Images
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Porcine CCL4 ELISA Kit standard curve
This standard curve is for demonstration only. A standard curve must be run with each assay.