PD-L1 antibody (28-8) IHC antigen retrieval and detection panel (ab236676)
Overview
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Product name
PD-L1 antibody (28-8) IHC antigen retrieval and detection panel
See all PD-L1 kits -
Species reactivity
Reacts with: Human -
Product overview
PD-L1 antibody (28-8) IHC antigen retrieval and detection kit ab236676 contains the 28-8 clone PD-L1 antibody (ab205921), and the critical reagents we recommend using with it for immunohistochemistry on paraffin-embedded tissue sections.
Kit contains:
ab205921 PD-L1 antibody (28-8)
ab208572 HIER antigen retrieval reagent
ab209101 Rabbit specific IHC polymer detection kit HRP
ab209101 contains amplifier secondary antibody, and HRP polymer tertiary antibody.
For instructions for use please consult the protocol books found on the online datasheets for ab205921, ab208572, and ab209101.
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Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
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Tested applications
Suitable for: IHC-P, IHC-Pmore details
Properties
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Storage instructions
Please refer to protocols. -
Components 1 units ab205921 - Anti-PD-L1 antibody [28-8] 1 x 50µl ab209101 - Rabbit specific IHC polymer detection kit HRP/DAB 2 x 15ml ab208572 - Universal HIER antigen retrieval reagent (10X) 1 x 100ml -
Research areas
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Function
Involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation and cytokine production. -
Tissue specificity
Highly expressed in the heart, skeletal muscle, placenta and lung. Weakly expressed in the thymus, spleen, kidney and liver. Expressed on activated T- and B-cells, dendritic cells, keratinocytes and monocytes. -
Sequence similarities
Belongs to the immunoglobulin superfamily. BTN/MOG family.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Cellular localization
Cell membrane and Endomembrane system. - Information by UniProt
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Alternative names
- B7 H
- B7 H1
- B7 homolog 1
see all -
Database links
- Entrez Gene: 29126 Human
- Omim: 605402 Human
- SwissProt: Q9NZQ7 Human
- Unigene: 521989 Human
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD-L1 antibody [28-8] (ab205921)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human non-small cell lung cancer tissue labelling PD-L1 with ab205921. Tumor cells and immuno cells localized within the stroma show PD-L1 plasma membrane staining.
For antigen retrival buffer, Universal HIER antigen retrieval reagent (ab208572) was used.
For IHC detection kit, Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) is recommended. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD-L1 antibody [28-8] (ab205921)](https://www.abcam.com/ps/products/236/ab236676/Images/ab236676-313330-pd-l1-antibody-28-8-ihc-antigen-retrieval-and-detection-panel-immunohistochemistry-formalin-paraffin.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD-L1 antibody [28-8] (ab205921)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human melanoma tissue labelling PD-L1 with ab205921 on Ventana Ultra. Tumor cells and immune cells show PD-L1 positive plasma membrane staining.
For antigen retrival buffer, Universal HIER antigen retrieval reagent (ab208572) was used.
For IHC detection kit, Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) is recommended.
