Recombinant cynomolgus monkey PD-L1 protein (Active) (ab221343)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level:
- Active: Yes
- Tags: His tag C-Terminus
- Suitable for: SDS-PAGE, Functional Studies
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Constituents: 95% PBS, 5% TrehaloseThis product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionReconstitute with sterile deionized water to a concentration of 400 µg/ml.
Images
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Functional Studies - Recombinant cynomolgus monkey PD-L1 protein (Active) (ab221343)
Immobilized ab221343 at 1 μg/mL (100 μL/well) can bind Anti-Human PD-L1 MAb with a linear range of 0.4-3 ng/mL.
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Functional Studies - Recombinant cynomolgus monkey PD-L1 protein (Active) (ab221343)Loaded Anti-Human PD-L1 MAb (Human IgG1) on AHC Biosensor, can bind ab221343 with an affinity constant of 19.1 nM as determined in BLI assay (ForteBio Octet Red96e).
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SDS-PAGE - Recombinant cynomolgus monkey PD-L1 protein (Active) (ab221343)ab221343 on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%. The protein migrates as 37-42 kDa under reducing condition due to glycosylation.
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Functional Studies - Recombinant cynomolgus monkey PD-L1 protein (Active) (ab221343)Anti-Human PD-L1 MAb captured on CM5 chip via anti-human IgG Fc antibody surface can bind ab221343 with an affinity constant of 23.3 nM as determined in a SPR assay (Biacore T200).
