Canine IL-6 ELISA Kit (ab193686)
Key features and details
- Sensitivity: 0.1 ng/ml
- Range: 0.1 ng/ml - 25 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Dog
Overview
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Product name
Canine IL-6 ELISA Kit
See all IL-6 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
0.1 ng/ml -
Range
0.1 ng/ml - 25 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 79.27 68% - 90% Plasma 78.06 69% - 91% Cell culture media 108 99% - 118% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Dog -
Product overview
Abcam’s IL-6 Canine ELISA Kit (ab193686) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of canine IL-6 in serum, plasma and cell culture supernatant.
This assay employs an antibody specific for canine IL-6 coated on a 96-well plate. Standards and samples are pipetted into the wells and the immobilized antibody captures IL-6 present in the samples. The wells are washed and biotinylated anti-canine IL-6 antibody is added. After washing away any unbound biotinylated antibody, an HRP-conjugated streptavidin is pipetted to the wells. After incubation, the wells are again washed, followed by the addition of a TMB substrate solution to the wells. Color will develop in proportion to the amount of IL-6 bound in each well. Addition of the Stop Solution will change the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent B 1 x 15ml 600X HRP-Streptavidin Concentrate 1 x 200µl Assay Diluent A 1 x 30ml Biotinylated canine IL-6 detection antibody (lyophilized) 2 vials Canine IL-6 Standards (lyophilized) 2 vials Pre-coated canine IL-6 microplate (12 strips x 8 wells) 1 unit Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Function
Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoeitic progenitor cells and cells of the CNS. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance. -
Involvement in disease
Genetic variations in IL6 are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ) [MIM:604302]. An inflammatory articular disorder with systemic-onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis.
Note=A IL6 promoter polymorphism is associated with a lifetime risk of development of Kaposi sarcoma in HIV-infected men. -
Sequence similarities
Belongs to the IL-6 superfamily. -
Post-translational
modificationsN- and O-glycosylated. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- Interleukin BSF 2
- B cell differentiation factor
- B cell stimulatory factor 2
see all -
Database links
- Entrez Gene: 403985 Dog
- SwissProt: P41323 Dog
Images
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Standard Curve.
Standard Curve in Assay Diluent A using ab193686 IL-6 Canine ELISA Kit.
Data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.
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Standard Curve.Standard Curve in Assay Diluent B using ab193686 IL-6 Canine ELISA Kit.
Data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.
