Plasma Kallikrein Assay Kit (ab241042)
Key features and details
- Detection method: Colorimetric
- Sample type: Plasma
Overview
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Product name
Plasma Kallikrein Assay Kit
See all Plasma Kallikrein 1B kits -
Detection method
Colorimetric -
Sample type
Plasma -
Product overview
Plasma Kallikrein Assay Kit (ab241042) utilizes the ability of active Plasma Kallikrein to cleave a synthetic pNA-based peptide substrate to release pNA (OD405 nm), which can be easily quantified using a microplate reader. The Plasma Kallikrein Specific Inhibitor (PKSI) selectively inhibits the ability of Plasma Kallikrein to the cleave the synthetic substrate. The kit is easy-to-use and can detect PK activity of Purified Plasma Kallikrein and Plasma Samples.
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests Human PK 1 vial PK Activator 1 x 1ml PK Assay Buffer 1 x 25ml PK Substrate 1 x 0.1ml PKSI Inhibitor 1 x 0.1ml pNA Standard (0.1 M) 1 x 20µl -
Research areas
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Function
The enzyme cleaves Lys-Arg and Arg-Ser bonds. It activates, in a reciprocal reaction, factor XII after its binding to a negatively charged surface. It also releases bradykinin from HMW kininogen and may also play a role in the renin-angiotensin system by converting prorenin into renin. -
Involvement in disease
Defects in KLKB1 are the cause of prekallikrein deficiency (PKK deficiency) [MIM:612423]; also known as Fletcher factor deficiency. This disorder is a blood coagulation defect. -
Sequence similarities
Belongs to the peptidase S1 family. Plasma kallikrein subfamily.
Contains 4 apple domains.
Contains 1 peptidase S1 domain. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- Fletcher factor
- kallikrein B plasma
- Kallikrein B plasma (Fletcher factor) 1
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Images
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Kinetic progressive curves.Kinetic progressive curves for different amounts of PK Enzyme.
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Kinetic progressive curves.Kinetic progressive curves for different amounts of Activated Plasma Samples are shown. -
Standard curve for pNA.Standard curve for pNA. -
PK activity.Standard curve for pNA (n = 3) was used to estimate PK activity in Normal Pooled Human Plasma (n = 3). Assays were performed according to the kit protocol.