Astana Biomed Group, an authorized Abcam distributor in Central Asia

Human CD83 ELISA Kit

(ab277711)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 2.32 pg/ml
  • Range: 9.38 pg/ml - 600 pg/ml
  • Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

Overview

  • Product name

    Human CD83 ELISA Kit

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Supernatant 8 4.7%
    Inter-assay
    Sample n Mean SD CV%
    Supernatant 3 3.2%

  • Sample type

    Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    2.32 pg/ml

  • Range

    9.38 pg/ml - 600 pg/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 104 100% - 106%
    Serum 104 98% - 108%
    Hep Plasma 99 88% - 111%
    EDTA Plasma 101 92% - 111%
    Cit plasma 98 89% - 106%

  • Assay time

    1h 30m

  • Assay duration

    One step assay

  • Species reactivity

    Reacts with: Human

  • Product overview

    Human CD83 ELISA kit (ab277711) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human CD83 protein in human serum, plasma, and cell culture supernatant samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human CD83 with 2.32 pg/mL sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            -Single-wash protocol reduces assay time to 90 minutes or less
            -High sensitivity, specificity and reproducibility from superior antibodies
            -Fully validated in biological samples
            -96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Notes

    CD83 is an activation marker for antigen presenting cells and is expressed on activated dendritic cells, interdigitating reticulum cells, and Langerhans cells. A soluble form of CD83 exists in serum that can inhibit maturation of dendritic cells. Elevated levels of CD83 are seen in autoimmune disease and hematological malignancies.

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Human CD83 Capture Antibody 1 x 600µl
    10X Human CD83 Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml
    Human CD83 Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent NS (ab193972) 1 x 12ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml

  • Research areas

  • Function

    May play a significant role in antigen presentation or the cellular interactions that follow lymphocyte activation.

  • Tissue specificity

    Expressed by activated lymphocytes, Langerhans cells and interdigitating reticulum cells.

  • Sequence similarities

    Contains 1 Ig-like V-type (immunoglobulin-like) domain.

  • Cellular localization

    Membrane.
  • Information by UniProt

  • Alternative names

    • B cell activation 45kDa cell surface glycoprotein Ig superfamily
    • B cell activation protein
    • B-cell activation protein
    • BL11
    • BL11 PEN
    • CD83
    • CD83 antigen
    • CD83 antigen (activated B lymphocytes, immunoglobulin superfamily)
    • CD83 molecule
    • CD83_HUMAN
    • Cell surface glycoprotein
    • Cell surface protein HB15
    • HB15
    • hCD83
    see all

  • Database links

    Images

    • SimpleStep ELISA Protocol Diagram
      SimpleStep ELISA Protocol Diagram

      SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

    • Example of human CD83 standard curve in Sample Diluent NS. The CD83 standard curve was prepared as described in Section 10.
      Example of human CD83 standard curve in Sample Diluent NS. The CD83 standard curve was prepared as described in Section 10.

      Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

    • Interpolated concentrations of native CD83 in human cell culture supernatant samples.
      Interpolated concentrations of native CD83 in human cell culture supernatant samples.

      The concentrations of CD83 were measured in duplicates, interpolated from the CD83 standard curves and corrected for sample dilution. Undiluted samples are as follows: HDLM-2 supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD83 concentration was determined to be 92.03 pg/mL in HDLM-2 supernatant.

    • Recombinant Antibody Benefits
      Recombinant Antibody Benefits

      To learn more about the advantages of recombinant antibodies see here.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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