Human CD83 ELISA Kit
(ab277711)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 2.32 pg/ml
- Range: 9.38 pg/ml - 600 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human CD83 ELISA Kit
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Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Supernatant 8 4.7% Inter-assay Sample n Mean SD CV% Supernatant 3 3.2% -
Sample type
Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
2.32 pg/ml -
Range
9.38 pg/ml - 600 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 104 100% - 106% Serum 104 98% - 108% Hep Plasma 99 88% - 111% EDTA Plasma 101 92% - 111% Cit plasma 98 89% - 106% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human CD83 ELISA kit (ab277711) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human CD83 protein in human serum, plasma, and cell culture supernatant samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human CD83 with 2.32 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
CD83 is an activation marker for antigen presenting cells and is expressed on activated dendritic cells, interdigitating reticulum cells, and Langerhans cells. A soluble form of CD83 exists in serum that can inhibit maturation of dendritic cells. Elevated levels of CD83 are seen in autoimmune disease and hematological malignancies.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human CD83 Capture Antibody 1 x 600µl 10X Human CD83 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml Human CD83 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
May play a significant role in antigen presentation or the cellular interactions that follow lymphocyte activation. -
Tissue specificity
Expressed by activated lymphocytes, Langerhans cells and interdigitating reticulum cells. -
Sequence similarities
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Cellular localization
Membrane. - Information by UniProt
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Alternative names
- B cell activation 45kDa cell surface glycoprotein Ig superfamily
- B cell activation protein
- B-cell activation protein
see all -
Database links
- Entrez Gene: 9308 Human
- Omim: 604534 Human
- SwissProt: Q01151 Human
- Unigene: 595133 Human
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human CD83 standard curve in Sample Diluent NS. The CD83 standard curve was prepared as described in Section 10.Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native CD83 in human cell culture supernatant samples.The concentrations of CD83 were measured in duplicates, interpolated from the CD83 standard curves and corrected for sample dilution. Undiluted samples are as follows: HDLM-2 supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD83 concentration was determined to be 92.03 pg/mL in HDLM-2 supernatant.
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Recombinant Antibody BenefitsTo learn more about the advantages of recombinant antibodies see here.
